Growth Characteristics and Influence of Antibiotics on Rough/Smooth Phenotypic Variants of Helicobacter pylori |
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Authors: | P A Bertram-Drogatz I Sobek-Klocke C Möller D Wingbermühle W Beil K-F Sewing M P Manns S Wagner |
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Institution: | (1) Institut für Allgemeine Pharmakologie, Medizinische Hochschule Hannover, D-30623 Hannover, Germany, DE;(2) Abteilung für Gastroenterologie und Hepatologie, Medizinische Hochschule Hannover, D-30623 Hannover, Germany, DE;(3) Mediport VC Management GmbH, Wiesenweg 10, D-12247 Berlin, Germany, DE |
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Abstract: | Helicobacter pylori shows a rather high variability of several biochemical markers including lipopolysaccharide structures. This study aimed
to determine whether Helicobacter pylori has a potential for phenotypic variability and to describe its effects on bacterial pathogenesis. From colonies of three
clinical strains of Helicobacter pylori with rough (R) colony morphology, spontaneous phenotypic variants with smooth (S) colony morphology were isolated that occurred
with a frequency of 10–2 to 10–3, irrespective of growth conditions. R-variant bacteria produced exclusively low-molecular-mass lipopolysaccharide. They exhibited
increased lysis in the presence of plain air. In contrast, the S variants produced low- and high-molecular-mass lipopolysaccharide
and did not exhibit increased lysis in the presence of plain air. Cocultivation of bacterial cells with AGS stomach cancer
cells revealed that R-variant bacteria but not S-variant bacteria effected an inhibition of high molecular-weight glycoprotein
biosynthesis and secretion by the host cells. Skirrow supplement added as selective agent to liquid and/or solid media was
tolerated to a similar extent among R- and S-variant bacteria, while all variants proved sensitive to metronidazole, amoxicillin
and clarithromycin except for the R and S isolates of strain Hp57, which showed resistance to the latter compound. It was
concluded that R- and S-variants of Helicobacter pylori may have distinct roles in pathogenesis; nevertheless, these bacteria may be isolated by traditional methods and eradicated
by conventional anti-infective therapy. |
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