8-羟基脱氧鸟苷在原发性翼状胬肉组织中的表达及意义

目的：检测DNA 氧化损伤标志物8-羟基脱氧鸟苷(8-OHdG)在翼状胬肉和正常结膜组织中的表达，探讨DNA氧化损伤在翼状胬肉发病机制中的作用。
　　方法：收集在我科行翼状胬肉切除手术的原发性翼状胬肉组织标本35例，并收集术眼颞上方正常球结膜标本5例作对照。采用免疫组织化学法检测翼状胬肉标本中8-OHdG的表达，并与正常球结膜组织的标本进行对照。
　　结果：在35例翼状胬肉组织中有24例呈阳性表达，阳性表达率为69%，而正常结膜组织中无8-OHdG的表达，其阳性表达率的差异具有显著统计学意义(P=0.007)。8-OHdG的阳性表达位于胬肉组织上皮细胞的细胞核，呈棕黄色着染，上皮下的纤维血管组织及正常结膜组织无表达。
　　结论：在翼状胬肉组织中8-OHdG呈阳性表达，而正常结膜组织中不表达，提示DNA氧化损伤在翼状胬肉的发病机制中发挥重要作用。

Expression and significance of 8-OHdG in primary pterygium

AIM:To detect oxidative DNA damage marker 8-hydroxy-2-deoxyguanosine(8-OHdG)in primary pterygium and normal conjunctival tissues, explore the role of oxidative DNA damage in the pathogenesis of pterygium.

METHODS: Totally 35 primary pterygium specimens were collected during surgery and 5 normal conjunctival specimens which above the normal temporal bulbar conjunctiva were collected. The expressions of 8-OHdG in pterygium tissues were detected by immunohistochemical method and compared with the normal conjunctival tissues. The difference of 8-OHdG expression between the two groups was compared.

RESULTS: There were 24(69%)pterygium specimens positive for 8-OHdG staining, limited to the nuclei of the epithelial layer. No substantial staining was visible in the subepithelial fibrovascular layers. All normal controls were negative for 8-OHdG staining. The difference of 8-OHdG expression between the two groups was statistically significant(P=0.007).

CONCLUSION: The increased levels of 8-OHdG in the pterygium tissues indicate that oxidative DNA damage maybe play an important role in the pathogenesis of pterygium.