血清中HCV抗体与HCV-RNA检测在临床中的应用

目的探讨丙型肝炎病毒抗体与RNA在临床中的应用价值。方法用ELISA法检测934例丙型肝炎可疑患者病毒抗体,用实时荧光定量PCR检测379例丙型肝炎可疑患者病毒RNA。结果934例患者HCV抗体阳性率为19.16%,379例HCV-RNA阳性率为43.01%。53例为两者均做的病人,RNA和Ab均阳性的占52.8%(28/53),均阴性占15.1%(8/53),两者的一致率达67.9%,Ab的阳性率为81.1%(43/53),RNA的阳性率为56.6%(30/53)。结论HCV抗体联合HCV-RNA检测对丙型肝炎的临床诊断有一定的价值。

Clinical Application of Blood Serum Anti - HCV and HCV - RNA Examination in Diagnosis of Patients With HCV

Objective To investigate the clinical value of serum anti-HCV and HCV-RNA in the diagnosis of hepatitis C patients. Methods Hepatitis C antibody was detected in 934 suspicious patients by ELISA, and HCV-RNA was detected in 379 suspicious patients by real time FQ-PCR. Results The positive rates of HCV antibody and HCV-RNA were 19.16% (179/934) and 43.01% (163/379) respectively. Fifty-three patients were tested HCV antibody and HCV-RNA; the positive rate of both of them was 52.8% (28/53), and the negative rate of both of them was 15.1% (8/53). The consistency rate was 67.9%, the positive rates of HCV antibody and HCV-RNA were 81.1% (43/53) and 56.6% (30/53) respectively. Conclusions Detection of antibodies against HCV and HCV-RNA plays an important role in clinical diagnosis of hepatitis C.