抗氧化剂预防低血糖所致肌肉损伤的研究

目的 探讨低血糖状态下血浆和组织中谷胱甘肽含量的变化以及还原型谷胱甘肽（GSH）对血清酶活性的抑制作用。方法 静脉注射胰岛素诱发低血糖，并持续60min，然后输注葡萄糖解除低血糖。结果发现诱发低血糖后6h，血浆GTSH，氧化型谷胱甘肽（GSSG），谷胱甘肽总量（TGSH）及GSSG/TGSH比值均明显升高，肝脏GSH和TGSH明显减少，心肌和骨骼肌CSSG和GSSG/TGSH比值明显增高，同时伴有血清酶（ALT，AST，LDH，CK）活性升高，预先注射CSH后诱发低血糖组，血浆GSSG和GSSG/TGSH比值明显下降，并抑制了血清酶的活性。结论 低血糖所致的血清酶活性的升高与体内谷胱甘肽氧化还原状态的改变有关。补充抗氧化剂GSH可以预防低血糖所致的肌肉损伤。

Antioxidant prevents muscle damages induced by experimental hypoglycemia

Objective To investigate the effect of hypoglycemia on glutathione levels in plasma and tissues and preventive effect of reduced glutathione (GSH) on serum enzymes. Methods In the model of hypoglycemia, changes in plasma and tissue contents of glutathione and inhibitory effects of reduced glutathione (GSH) on serum enzymes including alanine aminotransferase (ALT), aspartate aminotrasferase (AST), lactate dehydrogenase (LDH) and creatine kinase (CK) were observed in the study. Results Besides the increase of serum enzymes (ALT, AST, LDH, CK), plasma GSH, oxidized glutathione (GSSG), total glutathione (TGSH) and GSSG/TGSH ratio rose significantly ( P <0.05) at 6 hours after the induction of hypoglycemia. Significant decrease in contents of GSH and TGSH in liver and significant increases in GSSG contents and GSSG/TGSH ratio in myocardium and quadriceps were observed during hypoglycemia in contrast with control group. Administration of GSH significantly decreased the plasma GSSG levels and GSSG/TGSH ratio ( P <0.05), and suppressed the increases of serum enzymes induced by hypoglycemia. Conclusion The results suggest that increase of serum enzymes induced by hypoglycemia might be related to change of redox state of glutathione and that administration GSH may play a preventive role of muscle from damage.