大鼠睾丸支持细胞的微丝分布特征

采用TRITC—Phalloidin(鬼笔环肽)特异性染色和透射电镜方法,研究在体和离体培养的SD大鼠睾丸支持细胞的微丝分布规律。结果表明:在体支持细胞内微丝呈束状沿细胞膜下分布。在生精上皮基底部,微丝束聚集呈带状围绕于细胞基底部周缘,各束微丝相互平行;相邻支持细胞两侧膜下的微丝束排列走行基本对称一致。离体培养的支持细胞内微丝排布与细胞生活状态及铺展情况密切相关,在充分铺展细胞中微丝仍呈束状沿细胞膜下排列。证实沿细胞膜下成束排列是支持细胞内微丝分布的基本特征,这一特征对细胞整体形态及血一睾屏障的维持具有重要意义。

Distribution of Microfilaments in Rat Sertoli Cells in Vivo and in Vitro

The distribution of microfilaments (MFs) in rat Sertoli cells in vivo and in vitro was studied with electron microscopy and the TRITC-phalloidin staining method. The results revealed the following: The MFs of the Sertoli cells in vivo are arranged in bundles just beneath the cell membrane. MF bundles may form a band-like structure circumscribing the basal region of each Sertoli cell. The filaments and bundles are parallel with each other and their arrangement is symmetrical on the opposite sides of two adjacent Sertoli cell membranes, In vitro, the arrangement and distribution of MF bundles are closely related with the living and spreading state of the cell. In a fully spread cell, MFs bundles are arranged in parallel just beneath the cell membrane. We conclude that MFs in rat Sertoli cells are mainly arranged in bundles and stretched beneath the cell membrane. They may play an important role in maintaining the shape of the cell and the blood-testis barrier.