大鼠实验性脾气虚胃溃疡证病结合模型中大脑神经内分泌免疫网络的变化

目的：探讨脾气虚胃溃疡证病结合模型中大脑神经内分泌免疫网络(NEI)的变化及其作用。方法：采用免疫组织化学方法和放射免疫测定技术。结果：免疫组织化学检测结果为，脾气虚胃溃疡过程中大脑神经细胞分泌生长抑素、P物质、血管活性肠肽、白介素-2的活性均显著增强；放射免疫测定也显示下丘脑SST的分泌活性增强。结论：(1)大鼠实验性脾气虚胃溃疡期间，大脑局部神经、内分泌及免疫活动均发生了一定程度的变化。(2)高级神经中枢参与脾气虚合并胃溃疡疾病过程的调节。

Neuroendocrine-immunological changes in brain during experimental spleen-deficiency combined with gastric ulcer in rats

Objective: To explore the changes of neuroendocrine-immuno network in brain during experimental spleen-deficiency combined with gastric ulcer syndrome. Methods : Immunohistochemistry and radioimmunoassay were used. Results : Immunohistochemical study showed that the level of somatostatin(SST), substance P(SP), vascular active intestinal peptide (VIP), interleukin-2 (IL-2) secreted by neural cells in brain was higher during 4 and 14 days in spleen-deficiency combined with gastric ulcer. The level of SST in plasm and hypothalamus was also higher by radioimmunoassay. Conclusion :(1)The function of neuroendocrine-immuno has some changes in brain during experimental spleen-deficiency combined with gastric ulcer in rats. (2)Advanced neural center participates in the regulation during spleen-deficiency combined with gastric ulcer.