Growth characteristics of rhinoviruses in human gingival cells

Summary Type M and H rhinoviruses have been shown to replicate in human gingival tissue culture and under selective conditions to produce CPE. Both of these viruses replicate best at approximately 33° C with TCID₅₀/ml of 10⁵ and 10⁴ for rhinoviruses 2060 and 11,757, respectively.In gingival cells the type H rhinovirus (strain 11,757) has an eclipse phase from 1/2 to 6 hours and a maximum intracellular viral titer at 16 hours, whereas the type M rhinovirus (strain 2060) has an eclipse phase from 2 to 8 hours and a maximum intracellular viral titer at 24 hours. A more rapid viral replication of the type H virus was indicated by its rapid attachment to the host cell, short latent period and early virus release.This report was presented in part at the 46th General Meeting of the International Association for Dental Research, San Francisco, California, March 21–24, 1968.This investigation was supported by a General Research Support Grant awarded to the School of Dental Medicine, University of Pittsburgh by the National Institutes of Health, U.S. Public Health Service.