A Rapid and Simple Method for Electron Microscopy of Paraffin-Embedded Tissue |
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| Authors: | Sibylle Wid hn Lars-Gunnar Kindblom |
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| Affiliation: | Department of Pathology, Sahlgren Hospital, University of G?teborg, Sweden. |
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| Abstract: | A new rapid and simple method for electron microscopy of paraffin-embedded material is described. The paraffin-embedded tissue is deparaffinized in xylene, stained in a 0.01% toluidine blue/absolute ethanol solution, infiltrated in a propylene oxide/resin mixture, and embedded in epoxy resin. This quick method, which excludes rehydration, postosmification, and dehydration, takes about 3 h and produces results equal to previously described more laborious re-embedding techniques. |
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| Keywords: | electron microscopy paraffin-tissue re-embedding technique |
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