去松果体对大鼠学习能力及大脑皮质NOS表达的影响

目的探讨去松果体后其功能减退致褪黑素（ＭＴ）分泌减少对大鼠学习能力及大脑皮质一氧化氮合酶（ＮＯＳ）表达的影响。方法将大鼠进行Ｙ型迷宫测试，淘汰学习障碍的大鼠，将学习正常的大鼠随机分二组，实验组手术摘除松果体，对照组给予假手术，饲养４０天后再行Ｙ型迷宫测试，ＳＡＢＣ法检测神经元型一氧化氮合酶（ｎＮＯＳ）表达。结果实验组大鼠学习能力明显低于对照组大鼠（Ｐ〈０．０１），而大脑皮质、内侧隔核－斜角带核（Ｓ

Effects of pinealectomy on the learning abilities and the expression of nitric oxide synthase in cerebral cortex of rats

Objective To investigate the effects of decreased secretion of melatonin(MT) caused by pinealectomy on the learning abilities and the expression of nitric oxide synthase (NOS) in cerebral cortex of rats.Methods The rats were divided into two groups randomly after tested by Y type maze and those with learning dysfunction were eliminated,the experimental group was treated by pinealectomy, the control group was given sham operation. The rats were tested by Y type maze after fed for forty days and the expression of neuronal nitric oxide synthase (nNOS) was tested by SABC method.Results The learning abi lities of the experimental group were significant lower than those of the control rats ( P <0 01), nNOS positive cell in neocortex and medial septal nucleus diagonal band nucleus (SM DB) were more than that of control rats ( P <0.05 and P <0.01 respectively ),but there was no significant difference between the experimental group and the control rats in nNOS positive cell quantity of corpus striatum and hippocampus ( P >0.05).Conclusion Pinealectomy might cause the disorder of learning abilities in the rats,it might correlate with NO neurotoxicity arose by over expression nNOS in cerebral cortex and SM DB.