健脾益肾方对5/6肾切除大鼠肾组织α-SMA表达的影响

目的：探讨健脾益肾方治疗慢性肾衰竭（CRF）及其抗肾纤维化的作用机制,为临床应用提供实验依据。方法：将Wistar雄性大鼠随机分为正常对照组（N组）、模型组（M组）、低剂量治疗组（L组）、高剂量治疗组（H组）,除N组外均行5/6肾切除手术制作CRF动物模型。于造模后1周开始干预,干预8周后取血清及肾组织标本,全自动生化分析仪检测血清尿素氮（BUN）、肌酐（Scr）;免疫组织化学方法检测肾组织α-平滑肌肌动蛋白（α-SMA）的表达。结果：N组肾间质、肾小管周围、肾小管、肾小球无表达,肾间质血管、入球小动脉血管壁阳性;M组肾间质及肾小管上皮细胞强阳性表达,肾小球内亦有表达,与N组相比表达显著增强（P〈0.01）;L组及H组肾间质及肾小管上皮细胞中等强度表达,表达增多与N组相比有统计学差异（P〈0.01）,与M组相比表达显著降低（P〈0.01）;H组与L组相比表达显著降低（P〈0.05）。结论：健脾益肾方可以降低CRF大鼠血清BUN和Scr,抑制肾脏组织α-SMA的表达,表明健脾益肾方是治疗CRF的有效方剂,其机制可能与抑制大鼠肾组织内α-SMA等细胞因子的表达,从而抑制参与肾纤维化的细胞增殖和转分化,延缓肾纤维化的进展,达到治疗和延缓CRF进展的目的。

Influence of Jianpiyishen Decoction on Kidney α- SMA Expression in Rats with 5/6 Nephrectomy

Objective:To explore the action mechanism of Jianpiyishen Decoction on renal fibrosis and provide experimental evidence for clinical application.Methods:The male Wistar rats were divided into four groups randomly.There were normal control group(N group),model group(M group),low dose treatment group(L group) and high dose treatment group(H group).All the rats were established as CRF model with subtotal renal ablation except those in the N group.Interference was performed on the rats one week after the operat...