| 用套式PCR诊断肺炎支原体感染 |
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| 引用本文： | 顾兆坤,周弘,王志国,吴丽华,俞建康,陈巧林,黄达. 用套式PCR诊断肺炎支原体感染[J]. 江苏大学学报(医学版), 1997, 0(4) |
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| 作者姓名： | 顾兆坤 周弘 王志国 吴丽华 俞建康 陈巧林 黄达 |
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| 摘 要： | 应用套式PCR检测患儿咽部分泌物中的肺炎支原体（MP），阳性率为44．4％（28／63），比单式明显增高，由于两次扩增，增强信息量，增除生殖道支原体的假阳性，排除了其他干扰，提高检测的特异性、敏感性。
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| 关 键 词： | 套式PCR 支原体 肺炎 |
Detection of Mycoplasma Pneumoniae by using the nested Polymerase Chain Reaction |
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| Gu Zhaokun, Yu Jiangkang. Detection of Mycoplasma Pneumoniae by using the nested Polymerase Chain Reaction[J]. Journal of Jiangsu University Medicine Edition, 1997, 0(4) |
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| Authors: | Gu Zhaokun Yu Jiangkang |
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| Affiliation: | Deportment of Pediatrics of the Affiliated Hospital |
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| Abstract: | We have used the allele specific nested polymerase chain reaction to detect Mycoplasmapneumoniae from patient's throat swab samples, The positive rate is 49. 3%(28/63), aud is significantlybigher thau by Common PCR method. Because of being twice amplified, it contaius more iuformation, getsrid of the false positive of Mycoplasma genitalium, eliminates other interfereuces, and rises it'sspecificity and sensitivity in detectiug Mycoplasma, pneumoniae. |
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| Keywords: | nested polymerase chain reaction Mycoplasma Pneumoniae |
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