| 人胰岛素样生长因子-1重组腺病毒转染C17.2神经干细胞增殖及分化的影响 |
| |
| 引用本文： | 王莉梅,张东铭,沈庆煜,肖颂华,邢怡刚.人胰岛素样生长因子-1重组腺病毒转染C17.2神经干细胞增殖及分化的影响[J].医药论坛杂志,2009(3). |
| |
| 作者姓名： | 王莉梅 张东铭 沈庆煜 肖颂华 邢怡刚 |
| |
| 作者单位： | 郑州大学第一附属医院;郑州大学第二附属医院;中山大学第二附属医院; |
| |
| 摘 要： | 目的观察人胰岛素样生长因子-1重组腺病毒(Ad-IGF-1)转染对C17.2神经干细胞体外增殖和分化的影响。方法体外培养及鉴定C17.2神经干细胞,Ad-IGF-转染C17.2神经干细胞;Western-blotting法检测IGF-1蛋白的表达,酶联免疫吸附实验(ELISA)检测IGF-1蛋白的表达曲线;MTT检测C17.2神经干细胞增殖,NSE及MBP免疫组化观察C17.2神经干细胞体外分化。结果转染AdIGF-1的C17.2神经干细胞成功表达IGF-1蛋白;IGF-1蛋白在病毒转染5～7d达高峰,13d仍高于转染前,Ad-IGF-1转染后各时间C17.2神经干细胞的增殖与对照组相比有显著差异,Ad-IGF-1转染对c17.2神经干细胞向神经元细胞及神经胶质细胞的分化与对照组相比有显著差异。结论Ad-IGF-1转染c17.2神经干细胞可稳定表达IGF-1,Ad-IGF-1转染促进C17.2神经干细胞的增殖及向神经元细胞和少突胶质细胞的分化。
|
| 关 键 词： | 神经干细胞 IGF-1 增殖 分化 |
Effect of Transfection of Recombinant Adenovirus Containing IGF-1 Gene on Proliferation and Defferentiation of C17.2 Neural Stem Cell in Vitro |
| |
| WANG Limei,ZHANG Dongming,SHEN Qingyu,et al.Effect of Transfection of Recombinant Adenovirus Containing IGF-1 Gene on Proliferation and Defferentiation of C17.2 Neural Stem Cell in Vitro[J].Journal of Medical Forum,2009(3). |
| |
| Authors: | WANG Limei ZHANG Dongming SHEN Qingyu |
| |
| Institution: | WANG Limei,ZHANG Dongming,SHEN Qingyu,et al Department of neurology,The First Affiliated Hospital,Zhengzhou University,Zhengzhou 450052,China |
| |
| Abstract: | Objective To study the effects of transfection of recombinant adenovirus containing IGF-1 gene(Ad-IGF-1) on the proliferation and defferentiation of C17.2 neural stem cell in vitro.Methods C17.2 neural stem cell was cultured and identified in vitro,and was infected by Ad-IGF-1,the expression of IGF-1 was detected by Western blotting,the expression of IGF-1 at different time point was evaluated by enzyme linked immunosorbent assay(ELISA),the proliferation of C17.2 neural stem cell was detected by MTT,the def... |
| |
| Keywords: | Neural stem cell IGF-1 Proliferation differentiation |
| 本文献已被 CNKI 等数据库收录！ |
|
