Pokemon在肝细胞癌及癌旁、癌以远组织、肝硬化组织及正常肝组织中的表达

目的检测和分析Pokemon在肝细胞癌及癌旁组织、癌以远组织、肝硬化组织及正常肝组织中的表达。方法40例肝癌组织、距癌组织1cm以上的癌旁组织、距癌组织5 cm以上癌以远组织取自肝细胞癌合并乙型肝炎后肝硬化行肝移植患者切除病肝。20例肝硬化组织取自肝硬化行断流手术患者。20例正常肝组织取自健康供体标本。用real-time PCR(实时定量PCR,RT- PCR)法检测Pokemon蛋白mRNA在这些组织中的表达。结果肝癌组织、癌旁组织的Pokemon蛋白tuRNA的表达水平显著高于癌以远组织、肝硬化组织、正常肝组织(P<0.05)。肝癌组织与癌旁组织则差异无统计学意义(P>0.05)。癌以远组织、肝硬化组织及正常肝组织之间差异也无统计学意义(P>0.05)。结论Pokemon在肝细胞癌中存在异常高表达。癌旁组织可能存在着亦有Poke- mon蛋白mRNA高表达的的癌前病变。癌以远组织、肝硬化组织Pokemon蛋白mRNA表达水平与正常肝组织差异无统计学意义。

Expression of Pokemon in hepatocellular carcinoma tissue, adjacent and far tissue, hepatocirrhosis and normal liver tissue

Objective To detect the expression of Pokemon in hepatocellular carcinoma tissue, adjacent and far tissue, hepatocirrhosis and normal liver tissue. Methods Forty samples of hepatocellular carcinoma tissue, adjacent and far tissue were taken from the livers resected in liver transplantation for those with hepatocellular carcinoma and hepatocirrhosis. Twenty samples of hepatocirrhosis tissue were taken from the livers of those undergoing devascularization of fundus of stomach and cardia operation. Twenty samples of normal liver tissue were taken from the livers of donators for liver transplantation. The expression of Pokemon in all tissues was detected by real-time PCR. Results The expression of Pokemon in hepatocellular carcinoma and adjacent tissue, which had no significant difference, was markedly higher than that in far tissue, hepatocirrhosis and normal liver tissue. Conclusion The expression of Pokemon in hepatocellular carcinoma tissue is high. Precancerosis maybe exit in those tissues which are adjacent from hepatocellular carcinoma tissue with high expression of Pokemon.