胆管癌细胞系QBC939裸鼠肝门部胆管原位种植瘤模型的建立

目的建立胆管癌细胞系QBC939裸鼠肝门部胆管原位种植瘤模型并观察淋巴引流情况。方法应用我们前期建立的肝门部胆管癌细胞系QBC939,行裸鼠背部皮下接种,建立高转移特性胆管癌裸鼠皮下种植瘤模型,然后将高转移性种植瘤组织接种于裸鼠肝门部胆管与门静脉间组织间隙紧贴胆管处,4周及6周后行种植瘤瘤组织解剖学和病理学检查,并观察淋巴管引流情况。结果模型建立过程中,胆管癌细胞系QBC939细胞裸鼠皮下接种成瘤率为100%(10/10);原位种植4周后,肝门部胆管原位种植瘤成瘤率为100%(10/10),原位种植6周后,合并发生肝脏转移及腹腔淋巴结转移为80%(8/10)。结论成功建立了胆管癌细胞系QBC939裸鼠肝门部胆管原位种植瘤模型。

Establishment of human hilar cholangiocarcinoma cell line QBC939 orthotopic implantation tumor model in nude mice

Objective To establish human hilar cholangiocarcinoma cell line QBC939 orthotopic implantation tumor model in nude mice and observe the lymphatic drainages of the orthotopic implantation tumor.Methods The tumor mass which was formed by injecting human hilar cholangiocarcinoma cell line QBC939 subcutaneously was orthotopically transplanted into liver hilar of nude mice.Morphological and bi- ological features,invasion,metastasis and the lymphatic drainages of the transplanted tumor were studied after 4 and 6 weeks.Results The successful rate of hilar cholangiocarcinoma orthotopic transplantation was 100% after 4 weeks.The rate of metastasis of liver and lymphatic node was 80% after 6 weeks.Con- elusion Human hilar cholangiocarcinoma cell line QBC939 orthotopic implantation tumor model is suc- essfully established in nude mice.