浙江省1996～1999年急性弛缓性麻痹病例中非脊髓灰质炎肠道病毒分析

浙江省1996～1999年急性弛缓性麻痹（AFP）病例粪便标本中非脊髓灰质炎（脊灰）肠道病毒（NPEV）的平均分离率为11.2％。在67株NPEV中，柯萨奇病毒（Coxsackievirus，Cox.）B组21株，占31.3％；埃可病毒（EntricCytopothicHumanOrphanviruses，ECHO）25株，占37.4％；不能确定型别21株，占31.3％。NPEV按月分布显示，Cox.B病毒高峰期在5～7月，在1～3、10、12月未分离到；ECHO病毒全年均有，高峰期在8～11月。Cox.B病毒在Hep－2、RD细胞上平均滴度分别为10

Analysis of the Non -Polio Eneroviruses Isolated from AFP Cases in Zhejiang Province from 1996-1999

An average isolation rate of non-polio enteroviruses (NPEV) was 11.2% in Zhejiang Province during 1996-1999. There were 21 strains of Coxsackie B (31.3%), 25 strains of ECHO (37.4%) and 21 strains of unclassified virus (31.3%) isolsted from a total of 67 NPEV. The monthly distribution of the NPEV isolated from 1996 to 1999 was determined. Coxsackie B virus activity peaked from May to July, but none of the Coxsackie B virus was isolated from January to March and from both October and December. ECHO were present throughout the year and peaked from August to November. The titers of Coxsackie B viruses in Hep-2 and RD cells were 10 7.2 TCID 50 /ml and 10 4.6 TCID 50 /ml, while ECHO were 10 3.1 TCID 50 /ml and 10 6.9 TCID 50 /ml, respectively. There were some unclassified viruses, which could grow in L20B cells, but the CPE was different from that of the poliovirus and was similar to adenovirus.