体外培养的小鼠骨髓基质细胞对血细胞集落形成的支持

为探讨骨髓基质细胞在液体条件下支持造血的能力,对小鼠骨髓有核细胞体外培养,在液体培养基中形成基质细胞贴壁层,更换培养液,再种植骨髓有核细胞。结果在贴壁的基质细胞上形成了细胞集落。按细胞形态特征分为两类:一类为基质细胞集落,仅为偶见。另一类为造血细胞集落,数量较多,并且集落通过一定的结构与贴壁的基质细胞连接。结果提示,造血细胞集落的形成,需要有与骨髓基质细胞接触的微环境,并可对基质细胞的生长发育有一定的反作用。本文提出了造血细胞集落的液体培养法。

Assistance in Formation of Hemocyte to Colony by Mice Myeloid Matrix Cell Cultivate in Vitro

To study the hemotopoiesis capabitily supported by myeloid matrix cell in the liquid culture medium,the mice myelid kayocyte culture in vitro, after formation of sticking-wall layer of matrix cell in the liquid culture medium, changed culture solution and then replant myeloid karyocyte. Results showed that the cell colony was found on the stickingwall matrix cell. Morphologically, they can be classified into two groups, matrix cell colony and hematopoietic cell colony. The former could be seen occassionally while the latter appeared in great number. The conclusions are that the formation of hematopoietic cell colony require the micro-environment of myeloid matrix cells contact,and has a certain retroaction on the growth and development of matrix cell. The study created a liquid cultivation of hematopoietic cell colony.