| 8-Br-cAMP对肺癌NSCLC-3细胞凋亡相关基因表达的影响 |
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| 引用本文： | 陈奎生,欧阳松云,姜国忠,高冬玲,张云汉,马利军,孙培宗.8-Br-cAMP对肺癌NSCLC-3细胞凋亡相关基因表达的影响[J].郑州大学学报(医学版),2000,35(3):248-249. |
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| 作者姓名： | 陈奎生 欧阳松云 姜国忠 高冬玲 张云汉 马利军 孙培宗 |
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| 作者单位： | 1. 河南医科大学病理教研室,郑州,450052
2. 河南医科大学一附院呼吸内科,郑州,450052 |
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| 基金项目： | 河南省教委自然科学基金，1999320011， |
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| 摘 要： | 目的:观察8-Br-cAMP对人肺癌NSCLC-3细胞凋亡相关基因表达的影响.方法:将体外培育的NSCLC-3细胞分为实验组和对照组,采用原位杂交方法观察8-Br-cAMP对NSCLC-3细胞c-myc、bcl-2基因表达的影响.结果:8-Br-cAMP处理后的实验组较未加8-Br-cAMP的对照组c-myc、bcl-2基因表达降低(P＜0.01).结论:8-Br-cAMP可下调与NSCLC-3细胞凋亡相关的c-myc、bcl-2基因表达.
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| 关 键 词： | 8-Br-cAMP 细胞凋亡 原位杂交 |
Effects of 8-Br-cAMP on expression of related apoptotic gene in human lung cancer NSCLC-3 cell line |
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| CHEN Kuisheng,OUYANG Songyun,JIANG Guozhong,GAO Dongling,ZHANG Yunhan,MA Lijun,SUN Peizong.Effects of 8-Br-cAMP on expression of related apoptotic gene in human lung cancer NSCLC-3 cell line[J].Journal of Zhengzhou University: Med Sci,2000,35(3):248-249. |
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| Authors: | CHEN Kuisheng OUYANG Songyun JIANG Guozhong GAO Dongling ZHANG Yunhan MA Lijun SUN Peizong |
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| Abstract: | Aim: To observe the effects of 8 Br cAMP on expression of the related apoptotic gene in human lung cancer NSCLC 3 cells.Methods: The cultured NSCLC 3 cells in vitro were divided into the experimental and the control groups.The effects of 8 Br cAMP on expression of c myc and bcl 2 in NSCLC 3 cells were observed by in situ hybridization.Results:The expression of c myc and bcl 2 in the experimental group was weaker than that in the control group( P <0.01).Conclusion: 8 Br cAMP could suppress the related apoptotic gene expression of c myc and bcl 2 in NSCLC 3 cells. |
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| Keywords: | Br cAMP cell apoptosis in situ hybridization |
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