新疆哈萨克族和汉族食管癌组织ECRG2基因遗传多态性与易感性的关系

目的:研究食管癌相关基因2(ECRG2)短串联重复序列(STR)多态性与新疆哈萨克族和汉族食管癌易感性的关系。方法:新疆地区食管癌哈萨克族94例,汉族84例;人群对照哈萨克族100例,汉族53例,采用聚合酶链反应单链构象多态性分析(PCR-SSCP)技术检测研究对象的ECRG2 STR基因型。结果:ECRG2 STR呈多态性,可分为3种类型:TCA3/TCA3,TCA4/TCA4和TCA3/TCA4.3种基因型在有转移的食管癌中分布为58.8%,7.8%和33.3%,在无转移的食管癌中分布为14.2%,40.9%和44.9%,两者相比有显著差异(x~2=40.74,v=2,P<0.01,),携带TCA3/TCA3基因型个体更容易发生转移。汉族人群中,携带TCA3/TCA3基因型个体患食管癌的风险比TCA4/TCA4基因型个体高3.25倍(95%的可信区间为1.25-8.45);在哈萨克族人群中,携带TCA3/TCA3基因型个体患食管癌的风险比TCA4/TCA4基因型个体高4.06倍(95%的可信区间为1.69-9.74).结论:携带ECRG2基因TCA3/TCA3基因型个体与TCA4/TCA4基因型个体比较,增加了患食管癌和发生转移的风险。

Relationship between polymorphism of esophageal cancer related gene 2 and esophageal squamous cell cancer in Han and Kazakh populations in Xinjiang Uygur Autonomous Region

AIM:To investigate the relation between polymorphism of esophageal cancer related Gene(ECRG2)(short tandem repeat)STR and susceptibility in Xinjiang's esophageal squamous cell cancer(ESCC). METHODS:A case-control study was con- ducted with 178 case of esophageal cancer(94 of Kazakh and 84 of Han)and 153 populations (100 of Kazakh and 53 of Han)based controls in high incidence area of ESCC of Xinjiang.ECRG2 genotypes were detected by polymerase chain reactionsingle strand conformation polymor- phism(PCR-SSCP)method. RESU LTS:The frequencies of ECRG2 STR geno- types,TCA3/TCA3,TCA4/TCA4,TCA3/TCA4 were 58.8%,7.8% and 3.3% in metastasis esopha- geal cancer,and 14.2%,40.9% and 44.9% in cases who have no metastasis,respectively,There was statistical difference between the two groups (x~2=40.74,v=2,P<0.01).In both of the Han and Kazakh populations,subjects who carried the TCA3/TCA3 genotype were at an increased risk of ESCC compared to those carrying the TCA4/TCA4 genotype,with the adjusted odds ratios(ORs)being 3.25(95% CI,1.25-8.45)for the subjects of Hart population and 4.06(95% CI, 1.69-9.74)for the subjects of Kazakh population. CONCLUSION:Subjects who carried the TCA3/ TCA3 genotype are an increased risk of ESCC and metastasis compared to those carrying the TCA4/TCA4 genotype.