卵黄抗体液对幽门螺杆菌感染小鼠胃粘膜损伤的治疗作用

目的观察卵黄抗体液对幽门螺杆菌(Hp)感染小鼠胃粘膜损伤的治疗作用。方法给BABL/c小鼠采用灌胃法定植Hp,建立BABL/c小鼠感染胃炎动物模型。胃管灌胃前小鼠断食、断水12h,给予2%NaHCO3溶液0.2ml灌胃,1h后再给予Hp新鲜混悬菌液(109CFU)0.4ml灌胃,1h后恢复正常饮食,每两天重复一次,连续六次,造模成功后卵黄抗体治疗组分别灌服高、中、低剂量卵黄抗体液(0.6ml/0.4ml/0.2ml)1次/d,连续13d,正常对照组和胃炎模型组每只灌服生理盐水0.4ml1次/d,连续13d。距最后一次灌胃后8周,颈椎脱臼法处死小鼠,胃粘膜直接涂片、快速尿素酶试验及胃粘膜组织细菌培养检查小鼠Hp根除情况,并对胃窦部粘膜进行病理组织学检查。结果卵黄抗体液组分高、中、低剂量组的Hp根除率分别为80%、60%、40%,均明显高于模型组(P<0.05),同时胃窦部粘膜炎症较模型组均有不同程度的改善,组织切片Giemsa染色后卵黄抗体液组的Hp定植明显减少尤以高剂量组明显。结论卵黄抗体液组分具有较好的抗Hp感染和抗胃粘膜损伤作用并对胃炎有一定的治疗作用。

Therapeutic effect of yolk antibody fluids to gastric mucosal injury caused by Helicobacter pylori (Hp) infection in mice

Objective To observe the therapeutic effect of yolk antibody fluids to gastric mucosal injury caused by Helicobacter pylori (Hp) infection in mice. Methods BABL/c mice were used for model establishment of Hp infection, stayed without water or food for 12 hours before intragastric administration, and treated with 0.2ml of 2%NaHCO3 solution, one hour later, the mice were given 0.4ml of Hp fresh suspension bacterium fluids (103 CFU) and returned to normal diet one hour later. The experiment was repeated every two days once, six times consecutively. Then the mice were fed with yolk antibody fluids in a dose-dependent manner (0.6, 0.4, 0.2 ml/day) for 13 days. The normal control and gastritis model group were fed with saline solution. 8 weeks after the last intragastric administration, mice were autopsied. Urease test, bacterial culture of gastric mucosal tissue were used to determine and analyze the pathologic changes of gastric antrum mucosa. Results The eradiction rates of Hp in three different doses of yolk antibody fluids groups were 80%, 60%, 40% respectively, obviously higher than that in gastritis model group. The active inflammation of gastrivantrum mucosa was obviously improved in yolk antibody fluids groups than in gastritis model group. Giemsa staining showed that the localization of Hp in yolk antibody fluids was significantly decreased, especially in the high-dose group. Conclusion The yolk antibody fluids have the effect of anti-Hp infection, anti-injury of gastric mucosa and the therapeutical effect of gastritis.