| 氯化钴对神经型一氧化氮合酶在人神经母细胞瘤细胞表达的影响 |
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| 引用本文： | 李光宇,李英慧,王郡,吕晶玉,赵彦艳.氯化钴对神经型一氧化氮合酶在人神经母细胞瘤细胞表达的影响[J].解剖科学进展,2012,18(6):534-536,540. |
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| 作者姓名： | 李光宇 李英慧 王郡 吕晶玉 赵彦艳 |
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| 作者单位： | 1. 中国医科大学第一医院神经外科,辽宁沈阳,110001
2. 中国医科大学医学遗传学教研室,辽宁沈阳,110001 |
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| 基金项目： | 国家自然科学基金资助项目 |
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| 摘 要： | 目的研究CoCl2刺激后nNOS及其产物在人神经母细胞瘤细胞系SK-N-SH细胞中的表达变化。方法培养SK-N-SH细胞,用CoCl刺激细胞,Real-TimePCR检测nNOS mRNA表达变化,Western-blot检测蛋白表达,荧光法检测NO产量的变化。结果 CoCl2刺激后nNOS mRNA和蛋白在SK-N-SH细胞表达水平从刺激后12h开始表达增强,持续到48h,同时NO的生成量也明显升高。结论 CoCl2刺激引起SK-N-SH细胞nNOS表达水平及其产物NO明显升高。
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| 关 键 词： | 人神经母细胞瘤细胞系 神经型一氧化氮合酶 氯化钴 脑缺血 |
The effect of CoCl2 on nNOS expression in SK-N-SH cells |
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| LI Guang-yu
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LI Ying-hui
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WANG Jun
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LV Jing-yu
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ZHAO Yan-yan.The effect of CoCl2 on nNOS expression in SK-N-SH cells[J].Progress of Anatomical Sciences,2012,18(6):534-536,540. |
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| Authors: | LI Guang-yu LI Ying-hui WANG Jun LV Jing-yu ZHAO Yan-yan |
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| Institution: | 1.Department of Neurosurgery, the First affiliated Hospital, China Medical University; 2. Department of Medical Genetics, China Medical University, Shenyang 110001, China) |
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| Abstract: | Objective To study the effect of CoCl2 on the expression of nNOS in SK-N-SH cell line. Methods SK-N-SH cells were stimulated with CoCl , then real time-PCR and Western-blot were used to detect the expression level of nNOS mRNA and protein in SK-N-SH cells. DAF-2 DA fluorescence assay was used to detect the NO output. Results The expression level of nNOS mRNA and protein increased in SK-N-SH cells 12h after the stimulation of CoCl2 , continued until 48 hours after the stimulation, meanwhile the output of NO elevated obviously. Conclusion CoCl2 causes the increase of nNOS mRNA and protein and output of NO in SK-N-SH cell line. |
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| Keywords: | SK-N-SH cells neuronal nitric oxide synthase CoCl2 brain ischemia |
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