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流式细胞术在脑脊液白细胞检测上的应用
引用本文:朱峰.流式细胞术在脑脊液白细胞检测上的应用[J].中国医学装备,2008,5(12):47-49.
作者姓名:朱峰
作者单位:江苏省南京市江宁医院,江苏,南京,211100
摘    要:目的:探讨流式细胞术(FCM)在测定脑脊液标本白细胞数中的应用价值。方法:采用流式细胞术绝对计数法测定73份脑脊液标本的白细胞数,并与手工Neubauer计数盘计数结果相比较。结果:两种方法计数白细胞的结果具有高度相关性(r2=0.95),有显著性差异(P〈0.001),FCM法计数白细胞时仪器具有良好的线性。结论:流式细胞术绝对计数法因具有精确,客观性强的优点,是脑脊液白细胞计数的一种优选方法。

关 键 词:脑脊液  流式细胞术  白细胞

A flow cytometric method for the determination of leukocytes in cerebrospinal fluid
Zhu Feng.A flow cytometric method for the determination of leukocytes in cerebrospinal fluid[J].China Medical Equipment,2008,5(12):47-49.
Authors:Zhu Feng
Institution:Zhu Feng Jiangning Hospital of Jiangsu Province,Jiangsu Nanjing 211100,China.
Abstract:Objective:To study the application of flow cytometric absolute counting method for counting leukocytes in cerebrospinal fluid.Methods:Using flow cytometry method counts 73 samples of leukocytes in cerebrospinal fluid,with neubauer and hand-count results of the plate count compared.Results:Two kinds of methods correlated significantly(r2=0.95),and significant differences(P<0.001), FCM when the white blood count machines have good linear.Conclusion:The method of FCM provides a reliable and objective tool for measurement of countingleukocytes in cerebrospinal fluid.
Keywords:cerebrospinal fluid  Flow cytometry  leukocyte
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