| 早期干预对宫内感染致脑损伤仔鼠脑S-100蛋白及神经行为学的影响 |
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| 引用本文： | 庞伟,李晓捷,张士岭,郭岚敏. 早期干预对宫内感染致脑损伤仔鼠脑S-100蛋白及神经行为学的影响[J]. 实用儿科临床杂志, 2010, 0(2) |
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| 作者姓名： | 庞伟 李晓捷 张士岭 郭岚敏 |
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| 作者单位： | 佳木斯大学康复医学院儿童神经康复实验室; |
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| 基金项目： | 黑龙江省自然科学基金(D2004-50) |
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| 摘 要： | 目的研究早期干预对宫内感染致脑损伤仔鼠脑组织S-100蛋白表达和神经行为学的影响。方法孕第18天的Wistar大鼠连续2d腹腔注射脂多糖(LPS),制备宫内感染模型,9g·L-1盐水组给予同剂量9g·L-1盐水。随机选取9g·L-1盐水组仔鼠30只(对照组)和LPS组仔鼠50只。LPS组仔鼠随机分为干预组(n=20)和非干预组(n=30)。出生第1天非干预组和对照组各取10只大鼠,断头处死,取其脑组织,应用免疫组织化学方法测定其S-100蛋白水平,并在出生第25天对3组大鼠S-100蛋白水平行免疫组织化学检测和神经行为学检测。结果LPS组母鼠胎盘病理检测见胎盘内血管充血、水肿,并见大量中性粒细胞浸润,9g·L-1盐水组母鼠胎盘病理检测未见明显炎症反应;非干预组1日龄仔鼠脑组织各部位S-100蛋白阳性染色与对照组仔鼠比较,均有明显增加(Pa<0.01);25日龄仔鼠中,非干预组S-100蛋白表达最强,对照组最弱,干预组介于两者之间,各组间两两比较差异有统计学意义(Pa<0.01);非干预组仔鼠在悬吊试验、姿势反射、肌张力、不自主运动、旷场试验中的得分明显低于干预组和对照组,干预组明显高于非干预组(Pa<0.05...
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| 关 键 词： | 早期干预 脑损伤 脂多糖 S-100蛋白 |
Effects of Early Intervention on Expression of S-100 Protein and Neurobehavioral Change in Offspring Rats with Brain Injury Induced by Intrauterine Infection |
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| PANG Wei,LI Xiao-jie,ZHANG Shi-ling,GUO Lan-min. Effects of Early Intervention on Expression of S-100 Protein and Neurobehavioral Change in Offspring Rats with Brain Injury Induced by Intrauterine Infection[J]. Journal of Applied Clinical Pediatrics, 2010, 0(2) |
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| Authors: | PANG Wei LI Xiao-jie ZHANG Shi-ling GUO Lan-min |
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| Affiliation: | PANG Wei,LI Xiao-jie,ZHANG Shi-ling,GUO Lan-min(Children Neural Rehabilitation Laboratory,Rehabilitation Medicine College of Jiamusi University,Jiamusi 154002,Heilongjiang Province,China) |
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| Abstract: | Objective To research the effects of early intervention on the expression of S-100 protein and neurobehavioral change in offspring rats with brain injury induced by intrauterine infection.Methods Wistar rats in intrauterine infection group received lipopolysaccharide(LPS) intraperitoneal injection on the 18th day after getting pregnant,and the rats in normal saline group received the same dosage of normal saline.The placenta of rats in two groups were taken to detect the pathology after litter size.Thirty o... |
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| Keywords: | early intervention brain damage lipopolysaccharide S-100 protein |
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