Affiliation: | a Department of Clinical Chemistry, Danderyd Hospital, Karolinska Institute, Stockholm, Sweden b Institution of Infectious Diseases, Roslagstull Hospital, Karolinska Institute, Stockholm, Sweden c Institution of Pediatrics, St. Göran Hospital, Karolinska Institute, Stockholm, Sweden |
Abstract: | The zone immunoelectrophoresis assay (ZIA) for C-reactive protein (CRP) determinations is easy to perform and requires only small amount of antiserum, e.g., 25–100 and 0.5–1.0 μ1 anti-CRP antibody/20 serum and CSF samples, respectively. For quantitating CSF-CRP the immunoprecipitates formed were stained using lakaline phosphatase-conjugated secondary antibodies and the lowest standard concentration used was 30 μg/1. The immunoprecipitates formed when measuring CRP in serum were stained by Coomasie brilliant blue R250 with a detection limit of about 300 μg/l. CRP was determined in cerebrospinal fluid in 27 patients with bacterial meningitis (range <0.03–0.23 mg/l) and in 25 patients with viral meningitis (range <0.03–0.23 mg/l). CRP was quantitated in 52 sera by both the CRP ZIA method (y) and by electroimmunoassay (x). The correlation coefficient was r = 0.992 with the regression line y = 1.024 x + 0.855. |