电针对动情周期不同阶段大鼠GnRH免疫反应表达的研究

目的:探讨电针对下丘脑-垂体-靶腺轴特异性调节规律。方法:成熟雌性SD大鼠按动情周期的4个阶段随机分为假电针组、“关元”组、“内关”组。针刺治疗在早晨9∶30开始持续30min,下午15∶00时断头取脑组织。通过免疫组织化学染色观察促性腺激素释放激素(GnRH)免疫反应在下丘脑与生殖内分泌相关核团的表达。结果:①动情周期不同阶段GnRH免疫反应表达的数量不同,动情前期和动情期较多;②针刺后在内侧视前区、弓状核、室旁核等部位动情周期的不同阶段GnRH免疫反应的表达均有不同程度的增加,尤其是“关元”组在动情间期和动情后期GnRH“强”免疫反应表达增加明显。结论:电针后下丘脑GnRH免疫反应表达明显增加的部位有内侧视前区、弓状核、室旁核等,“关元”组优于“内关”组,动情间期及动情后期表现明显。

Study on effects of electroacupuncture on the expression of GnRH in the rat at different estrous cycles

OBJECTIVE: To explore the specific regulative law of electroacupuncture (EA) for the hypothalamus-pituitary-gonad axis. METHODS: The adult female SD rats were divided into a simulant EA group, Guanyuan (CV 4) group and Neiguan (PC 6) group according to 4 stages of the estrous cycle. Acupuncture was given from 9 : 30 AM to 10 : 00 AM and the brain tissue samples were collected at 15 : 00 PM. GnRH expressions in the nuclei related with genital neuroendocrine in the hypothalamus were investigated with immunohistochemical method. RESULTS: (1) The GnRH expression quantity was different in different estrous cycles in the simulant EA group, with more expression in the proestrus and the estruation; (2) After acupuncture, the GnRH expressions in the medial preoptic area, the arcuate nuclei and the nuclei periventriculares were increased in different stages of the estrous cycle in varying degrees, with the "strong" GnRH expression increased significantly in the diestrus and the metaoestrus in the Guanyuan (CV 4) group. CONCLUSION: After EA, the expression of the GnRH-positive reactant in the medial preoptic area, the arcuate nuclei and the nuclei periventriculares of the hypothalamus significantly increases, with the Guanyuan (CV 4) group better than the Neiguan (PC 6) group, and more obvious expressions in the diestrus and the metaoestrus.