| 基于高分离度和对照图谱的红参中9种人参皂苷类成分“一测多评”质量评价研究 |
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| 引用本文： | 冯伟红,李春,吉丽娜,傅欣桐,朱晶晶,陈两绵,杨立新,荣立新,张永欣,王智民.基于高分离度和对照图谱的红参中9种人参皂苷类成分“一测多评”质量评价研究[J].中国中药杂志,2016,41(18):3389-3399. |
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| 作者姓名： | 冯伟红 李春 吉丽娜 傅欣桐 朱晶晶 陈两绵 杨立新 荣立新 张永欣 王智民 |
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| 作者单位： | 中国中医科学院 中药研究所, 北京 100700;中药质量控制技术国家工程实验室, 北京 100700,中国中医科学院 中药研究所, 北京 100700;中药质量控制技术国家工程实验室, 北京 100700,中国中医科学院 中药研究所, 北京 100700;中药质量控制技术国家工程实验室, 北京 100700;天津市药品检验所, 天津 300070,北京市药品检验所, 北京 102206,中国中医科学院 中药研究所, 北京 100700;中药质量控制技术国家工程实验室, 北京 100700,中国中医科学院 中药研究所, 北京 100700;中药质量控制技术国家工程实验室, 北京 100700,中国中医科学院 中药研究所, 北京 100700;中药质量控制技术国家工程实验室, 北京 100700,中国中医科学院 广安门医院, 北京 100053,中国中医科学院 中药研究所, 北京 100700;中药质量控制技术国家工程实验室, 北京 100700,中国中医科学院 中药研究所, 北京 100700;中药质量控制技术国家工程实验室, 北京 100700 |
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| 基金项目： | 国家药品标准提高暨2015版药典科研任务；中药质量标准研究和信息化体系建设平台项目（2012ZX09304005）；国家“重大新药创制”科技重大专项（2014ZX09304307001-002-007） |
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| 摘 要： | 采用反相高效液相色谱法,Ultimate~C_(18)色谱柱,对红参中9种人参皂苷类成分进行了具有高分离度和高色谱峰纯度的同步色谱分离。采用"一测多评"中药质量评价模式,经一系列方法学考察,以人参皂苷Rb_1为内参物,建立了人参皂苷Rg_1,Re,Rf,Rb_1,Rc,Rb_2,Rb_3,Rd和20(S)-人参皂苷Rg3与人参皂苷Rb1间的相对校正因子与相对保留值,在红参对照图谱的辅助定位下,最终实现了仅采用人参皂苷Rb1一个中药对照品对红参中上述9种人参皂苷类成分的同步质量控制。
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| 关 键 词： | 红参 一测多评 高分离度 高色谱峰纯度 对照图谱 9种 人参皂苷 |
| 收稿时间： | 7/7/2016 12:00:00 AM |
Quantitative analysis of nine ginsenosides in red ginseng using QAMS method based on high-resolution and reference chromatography of red ginseng |
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| FENG Wei-hong,LI Chun,JI Li-n,FU Xin-tong,ZHU Jing-jing,CHEN Liang-mian,YANG Li-xin,RONG Li-xin,ZHANG Yong-xin and WANG Zhi-min.Quantitative analysis of nine ginsenosides in red ginseng using QAMS method based on high-resolution and reference chromatography of red ginseng[J].China Journal of Chinese Materia Medica,2016,41(18):3389-3399. |
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| Authors: | FENG Wei-hong LI Chun JI Li-n FU Xin-tong ZHU Jing-jing CHEN Liang-mian YANG Li-xin RONG Li-xin ZHANG Yong-xin and WANG Zhi-min |
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| Institution: | Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicines, Beijing 100700, China,Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicines, Beijing 100700, China,Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicines, Beijing 100700, China;Tianjin Institute for Drug Control, Tianjin 300070, China,Beijing Institute For Drug Control, Beijing 102206, China,Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicines, Beijing 100700, China,Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicines, Beijing 100700, China,Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicines, Beijing 100700, China,Guang''anmen Hospital, China Academy of Chinese Medical Sciences, Beijing 100053, China,Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicines, Beijing 100700, China and Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicines, Beijing 100700, China |
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| Abstract: | Using reversed-phase high performance liquid chromatography, nine ginsenosides were simultaneously separated on an Ultimate®C18 column with high-resolution and high purity of each chromatographic peak. Adopting the QAMS quality evaluation model for traditional Chinese medicines, ginsenoside Rb1 was used as the internal reference substance, and the relative correction factors (RCFs) and the relative retention values (RTRs) of ginsenosides Rg1, Re, Rf, Rb1, Rc, Rb2, Rb3, Rd and 20 (S)-ginsenoside Rg3 to ginsenoside Rb1 were calculated individually. Through a series of methodology evaluations, and positioned by the red ginseng reference chromatograph and RTVs, nine ginsenosides in red ginseng were simultaneously assayed only by quantitative determined ginsenoside Rb1. |
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| Keywords: | red ginseng QAMS (qualitative assay of multi-components by a single reference substance) high-resolution high purity reference chromatogram ginsenosides |
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