双向固体发酵对板蓝根和大青叶抑菌的增效作用初探

目的探讨25种不同食(药)用真菌菌株对板蓝根和大青叶的双向固体发酵的转化作用,得到具有较好抑菌作用的发酵菌质产物。方法经过板蓝根和大青叶平板初筛法和三角瓶复筛法,获得能在大青叶和板蓝根上生长的优势菌株;进一步通过抑菌实验和薄层色谱(TLC)实验,确定发酵得到的菌质产物的抑菌作用。结果复筛得到能够在板蓝根(灵芝11、灵芝13、灵芝7)和大青叶(灵芝2、灵芝11、灵芝3)上生长良好的菌株,其中含灵芝3和灵芝11的发酵液与空白对照相比具有较好的抑菌作用。TLC结果显示,灵芝2发酵大青叶的甲醇提取物有蓝色斑点为发酵后新增成分。结论板蓝根和大青叶经药用真菌进行双向固体发酵后能够有效提高其抑菌作用,而且有新物质产生。

Study on synergism of bidirectional solid fermentation on antibacterial effect of Isatidis Radix and Isatidis Folium

Objective To explore the bidirectional solid fermentation transformation of 25 different edible fungal strains with Isatidis Radix(IR) and Isatidis Folium(IF) to get the synergism antibacterial effect of fermentation substance.Methods The dominant strains grown on IR and IF were obtained through initial screening and repeated screening.The antibacterial effect of fermentation product substance were determined by antibacterial test and TLC.Results The results showed that repeated screening got three strains which could grow fast in IR(G-11,G-13,and G-7) and IF(G-2,G-11,and G-3).The G-3 strain and G-11 strain fermentation substance had better antibacterial effect than IR and IF respectively.The methanol extract of G-2 fermented IF substance appeared a new spot by TCL,it should be an additional component after fermentation.Conclusion The bidirectional solid fermentation could improved antibacterial effect of IR and IF,and the new active ingredients can be produced.