金线莲丛生芽培养及有效物质生产研究

目的探明3种因素对金线莲Anoectochilus roxburghii丛生芽生物量和有效物质积累的影响以及在反应器内通过大量培养丛生芽生产有效物质的可行性,为金线莲产品开发提供一种新的材料来源。方法以金线莲丛生芽为材料,研究外植体长度、BA质量浓度和光条件对金线莲丛生芽的影响,观察利用气升式生物反应器培养金线莲丛生芽对生物量和有效物质积累的影响。结果外植体大小为5 mm时丛生芽增殖效果显著好于1 mm。BA质量浓度为2.5 mg/L时生物量最高,但丛生芽中多糖、黄酮和总酚等有效物质的生产量在BA质量浓度为2.0 mg/L时出现最高值。明培养比暗培养更适于生物量和有效物质的积累。反应器培养的丛生芽生物量,多糖,黄酮和酚类物质的生产量(678.3、12.2、17.8 mg/L)明显高于固体培养。结论外植体长度为5 mm、BA质量浓度为2.0 mg/L、在明培养条件下,利于丛生芽的生长,可生产较多的多糖、黄酮和酚类物质。在短时间内通过气升式生物反应器可得到大量丛生芽,反应器培养比固体培养能获得更多的生物量和有效物质。

Multiple shoot culture and effective material production of Anoectochilus roxburghii

Objective To provide a new material for exploring the products of Anoectochilus roxburghii,and study the effect of explant lengths,BA concentration and light intensities on multiple shoot biomass and effective material accumulation of A.roxburghii;This paper also explored the feasibility of the bioreactor culture on the rapid proliferation of A.roxburghii.Methods The optimum explant lengths for multiple shoot proliferation were selected,the effect of different BA concentration and light intensities on multiple shoot biomass and effective material accumulation were investigated.Additionally,the comparison was carried out between bioreactor culture and conventional solid culture for multiple shoot biomass and effective material accumulation of A.roxburghii.Results Explants(5 mm) promoted multiple shoot proliferation,mutiple shoot biomass was significantly higher than explants(1 mm).MS medium supplemented with 2.5 mg/L BA was optimal for multiple shoot biomass accumulation but the maximum productivities of polysaccharide,flavonoid,and total phenolic were found at 2.0 mg/L BA.Multiple shoot biomass and effective material accumulation were more suitable under light condition than under dark condition.The biomass and production of polysaccharide,flavonoid,and total phenolic(678.3,12.2,and 17.8 mg/L) of multiple shoot at 5 g/L of inoculation density were significantly better than in solid culture.Conclusion Multiple shoot biomass and productivities of polysaccharide,flavonoid,and phenolic compound are enhanced when 5 mm explants are inoculated in MS medium supplemented with 2.0 mg/L BA and cultured under light condition.In addition,bioreactors are used for large scale propagation of multiple shoot in less time.The accumulation of biomass and bioactive compound during bioreactor culture is higher than in solid culture.