降脂通络软胶囊对膜性肾病大鼠肾保护作用及对肾组织Bcl-2和Bad表达的影响

目的观察降脂通络软胶囊对膜性肾病大鼠模型血液生化指标及对肾组织Bcl-2、Bad表达的影响,探讨降脂通络软胶囊对膜性肾病大鼠的肾保护作用和可能机制。方法将60只SD大鼠随机选取10只为对照组,其余50只进行造模,造模成功后将大鼠随机分为模型组、贝那普利组和降脂通络软胶囊(25、50、100 mg/kg)组,每组各10只,各组按相应剂量给药,在给药的第4周末检测大鼠24 h尿蛋白定量(UTP)及血清总胆固醇(TC)、三酰甘油(TG)、总蛋白(TP)、白蛋白(ALB)、尿素氮(BUN)、肌酐(Scr)水平;采用电镜、免疫荧光观察肾脏病理形态学变化,免疫组化及Real-time PCR法检测各组大鼠肾组织Bcl-2、Bad的表达情况。结果与模型组比较,各给药组UTP、TC、TG均有所降低(P0.05),各给药组TP、ALB均有所升高(P0.05)。降脂通络软胶囊50、100 mg/kg组与贝那普利组UTP、TC、TG、TP、ALB比较差异不显著(P0.05),效果优于降脂通络软胶囊25 mg/kg组(P0.05)。与对照组比较,各给药组及模型组BUN、Scr均未见明显变化,差异不显著(P0.05)。与模型组相比,各给药组大鼠肾组织中Bcl-2 mRNA的表达较模型组明显上调,Bad mRNA表达下调,差异显著(P0.05);降脂通络软胶囊50、100 mg/kg组与贝那普利组Bcl-2、Bad比较差异不显著(P0.05),效果优于降脂通络软胶囊25 mg/kg组(P0.05)。结论降脂通络软胶囊对膜性肾病大鼠的肾保护作用可能与其上调肾组织中Bcl-2 mRNA表达,下调Bad mRNA表达,抑制足细胞凋亡、修复受损足细胞有关。

Renal protection of Jiangzhi Tongluo Soft Capsule on membranous nephropathy rats and effects on expression of Bcl-2 and Bad in renal tissue

Objective To observe the effects of Jiangzhi Tongluo Soft Capsule on blood biochemical indexes and expression of Bcl-2 and Bad in membranous nephropathy(MN) rats,explore the renal protective effects and possible mechanism of Jiangzhi Tongluo Soft Capsule on MN rats.Methods Total 60 SD rats were randomly selected,10 for normal group,the other 50 for MN model establishment,then randomly divided into model group,Benazepril group,and Jiangzhi Tongluo Soft Capsule group(25,50,and 100 mg/kg),each group was treated according to the corresponding dose.The 24 h urinary protein quantitative(UTP),serum total cholesterol(TC),triglyceride(TG),total protein(TP),albumin(ALB),blood urea nitrogen(BUN),and serum creatinine(Scr) in rats in the 4th weekend were tested;The renal pathological morphology changes were observed by electron microscope and immunofluorescence technique,and detecting the expression of Bcl-2,Bad by immunohistochemical and real-time PCR methods.Results Compared with the model group,UTP,TC,and TG in the treatment groups were lower(P<0.05),TP and ALB in the treatment groups were increased(P<0.05),and the differences of UTP,TC,TG,TP and ALB in Jiangzhi Tongluo Soft Capsule 50,100 mg/kg groups,and Benazepril group have no statistical significance(P>0.05),and the effect was better than Jiangzhi Tongluo Soft Capsule 25 mg/kg group(P<0.05).Compared with the normal group,the difference of BUN and Scr in the treatment groups and model group has no statistically significant(P>0.05).Compared with the model group,the expression of Bcl-2 mRNA significantly raised(P<0.05),and the expression of Bad mRNA was lower(P<0.05);There was no statistically significant difference compared with Benazepril group and Jiangzhi Tongluo Soft Capsule 50 and 100 mg/kg groups(P>0.05),and the effect was better than that in Jiangzhi Tongluo Soft Capsule 25mg/kg group(P<0.05).Conclusion The renal protection of Jiangzhi Tongluo Soft Capsule on MN rats may be related to the function of raising the expression of Bcl-2,downgrading the expression of Bad,inhibiting the sertoli cell apoptosis,and repairing the damaged cells.