The synthesis of lipids from [1-14C]acetate by isolated rat brain capillaries |
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Authors: | P Homayoun F Roux E Niel J M Bourre |
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Affiliation: | INSERM-Unité 26, Hôpital Fernand Widal, 200, rue du Faubourg St-Denis, F-75475 Paris Cedex 10 France |
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Abstract: | Lipid biosynthesis was investigated in isolated cerebral microvessels obtained from adult Sprague-Dawley rats using [1-14C]acetate as precursor. All lipid classes were labelled by [1-14C]acetate. Neutral lipids incorporated about 50% of radiolabelled acetate, among which free fatty acids and triglycerids showed the highest level of incorporation. Moreover, about 4% of radioactivity was found in cholesterol fraction. In phospholipid fraction, phosphatidylcholine and phosphatidylethanolamine were the main radiolabelled phospholipids. [1-14C]acetate was also incorporated into sulphatides and cerebrosides. The presence of bovine serum albumin in incubation medium modified the percentage of incorporation in different lipid fractions. |
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Keywords: | lipid synthesis brain capillary rat |
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