| 应用PCR检测HL－60细胞诱导分化前后c－myc癌基因的扩增 |
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| 引用本文： | 张婕,王芸庆,孙开来. 应用PCR检测HL－60细胞诱导分化前后c－myc癌基因的扩增[J]. 中国医科大学学报, 1996, 0(5) |
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| 作者姓名： | 张婕 王芸庆 孙开来 |
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| 作者单位： | Department of Cell Biology，School of Basic Medical Sciences，China Medical University，Shenyang，110001 |
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| 摘 要： | 应用ＰＣＲ方法研究了维甲酸（ＲＡ）和二甲基亚砜（ＤＭＳＯ）诱导ＨＬ－６０细胞分化过程中ｃ－ｍｙｃ基因扩增的变化。结果：ＨＬ－６０细胞内ｃ－ｍｙｃ基因扩增为１１，经ＲＡ诱导６ｄ后，其扩增程度无明显变化，而ＤＭＳＯ诱导３ｄ后，ｃ－ｍｙｃ基因扩增出现下降，至５ｄ，较未诱导分化前下降约３０％。提示了ＲＡ和ＤＭＳＯ对ＨＬ－６０细胞不同的诱导分化机制。
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| 关 键 词： | c－myc基因；人早幼粒细胞白血病细胞系HL－60；诱导分化；聚合酶链反应 |
Detection of c myc Oncogene Amplification During Induced Differentiation of HL 60 Cells |
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| Zhang Jie,Wang Yunqing,Sun Kailai. Detection of c myc Oncogene Amplification During Induced Differentiation of HL 60 Cells[J]. Journal of China Medical University, 1996, 0(5) |
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| Authors: | Zhang Jie Wang Yunqing Sun Kailai |
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| Abstract: | olymerase chain reaction(PCR) was used to study c myc oncogene amplification of HL 60 cells during the differential induction by retinoic acid(RA) and dimethyl sulfoxido(DMSO).C myc gene is amplified about 11 fold in HL 60 cells. The amplification has no significant change after six days induction by RA while three days after DMSO induction,some c myc gene loss was observed. About 30% loss of myc gene was observed on the 5 th day of DMSO induction. This result suggests the different action of RA and DMSO on HL 60 induced differentiation. |
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| Keywords: | c myc gene HL 60 differential induction PCR |
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