姜黄素对荷瘤裸鼠皮下移植瘤的影响

目的观察姜黄素在体内对人肝癌细胞株SMMC-7721的抗肿瘤作用。方法用姜黄素在SMMC-7721肝癌细胞荷瘤裸鼠的瘤体内进行注射治疗,12 d后处死裸鼠,摘除瘤体,称瘤重,观察肿瘤生长变化,并通过免疫组化法检测Bcl-2、Bax、Caspase-3等与细胞凋亡相关因子的表达。结果姜黄素可抑制SMMC-7721细胞对裸鼠的致瘤能力,肿瘤体积较对照组显著减小(P0.01),质量也明显小于对照组(P0.01),肿瘤生长抑制率达47.7%,免疫组化结果显示阿的平能明显上调与细胞凋亡相关因子Bax和Caspase-3的表达和下调Bcl-2和Survivin的表达。结论姜黄素能抑制SMMC-7721细胞的体内致瘤能力。

Effect of curcumin on subcutaneous transplantation tumor of human hepatoma in nude mice

Objective To study the anti-tumor effects of curcumin on human hepatocellular carcinoma SMMC-7721 Cells in vivo.Methods For in vivo study,we first established SMMC-7721 tumor model by grafting SMMC-7721 cells in athymic nude mice,and then injected curcumin into the tumors.12 days after injection,we sacrificed the mice,removed the tumors,weighed and calculated the ratios of tumor-suppression.We also detected the expressions of Bcl-2,Bax,Caspase-3 and Survivin with immumohistochemistry.Results The tumorigenicity of SMMC-7721 cells was significantly inhibited by curcumin.The size of the tumor in the curcumin treatment group was significantly smaller than that in the control group(P<0.01),and the tumor weight was also markedly reduced in curcumin group than that in the control group [(0.485±0.178) g vs(0.928±0.223) g,(P<0.01)].The tumor growth inhibition rate was 47.7%.The in vivo data showed that curcumin suppressed the tumor growth conspicuously through down-regulating the expressions of bcl-2 and Survivin and up-regulating the expressions of bax and caspase-3.Conclusion Curcumin inhibits the in vivo tumorigenicity of SMMC-7721 cells.