应用扩增受阻突变检测系统（ARMS）检测HBV基因型

目的建立扩增受阻突变检测系统（ARMS）检测乙型肝炎病毒（HBV）基因型方法，并对慢性乙型肝炎（CHB）患者HBV基因型进行分析。方法通过对HBV全基因组序列比对分析，设计ARMS特异性引物和探针体系，建立检测HBVB和C基因型方法，对50例临床标本进行检测。结果50例临床样本中，B型的检出率为26％（13例），C型为74％（37例），其实验结果与测序结果完全一致。常州及周边地区乙型肝炎病毒C基因型HBV为优势株。C基因型患者A1762T／G1764A突变率为62％，明显高于B型。结论ARMS检测HBV基因型，快速、准确；常州及周边地区HBV基因型主要为B、C型，且C型携带A1762T／G1764A双突变率较高，与较为严重的肝病相关。

Detection of hepatitis B virus genotypes by amplification refractory mutation system

Objective To Establish amplification refractory mutation system （ARMS） for hepatitis B virus genotyping assay and detect HBV genotype in patients with chronic hepatitis B by it. Method Based on HBV genome sequence alignment, we designed specific primers and probe, established ARMS for HBV B and C genotyping and detected 50 clinical samples with chronic HBV infection. Results Among 50 samples, genotype B and C which were detected by ARMS assay is 26% （14/50） and 74% （37/50） separately. The results were completely consistent with sequencing results. Genotype C was more prevalent than genotype B in Changzhou area, and 62% patients with genotype C carried A1762T/G1764A double mutation, much higher than genotype B. Conclusion ARMS was a fast and accurate assay for HBV genotype detection. Dominant HBV genotypes in Changzhou area were genotypes B and C, and genotype C carried more A1762T/G1764A double mutation, maybe associated with more sever liver disease.