SYSMEX XN-1000血球仪在脑脊液和胸腹水白细胞计数中的可行性探讨

目的：探讨SYSMEX XN-1000血液分析仪(简称SYSMEX XN-1000)的体液模式在脑脊液和胸腹水白细胞计数中应用的可行性。方法分别采用手工显微镜镜检法和SYSMEX XN-1000对183例脑脊液和胸腹水标本进行白细胞计数，将所测结果按A：(0~1000)×106/L，B：(1001~5000)×106/L，C：>5000×106/L分为三组，分别进行统计学分析。结果与手工显微镜镜检法相比，SYSMEX XN-1000所测结果差异无统计学意义(P>0.05)。A、B、C三组的相关系数分别为0.993、0.984、0.996，A组回归方程为y=1.007x-1.072，B组回归方程为y=1.003x+19.776，C组回归方程为y=1.241x-1419.365。结论 SYSMEX XN-1000血液分析仪可用于临床脑脊液和胸腹水中白细胞计数，其操作简单易行，结果稳定可靠，但如果仪器进行样品检测时报警，应当手工镜检复核，以提高结果的准确性和可靠性。

Feasibility study of white blood cell count in cerebrospinal fluid and hydrothorax and ascites with Sysmex ;XN-1000 hematology analyzer

Objective To analyze the feasibility of using body fluid module of Sysmex XN-1000 hematology analyzer for white blood cell count in the cerebrospinal fluid (CSF), hydrothorax and ascites. Methods Microscope method and Sysmex XN-1000 hematology analyzer were used to analyze 183 samples of CSF, hydrothorax and ascites at the same time. The measured results were divided into three groups:group A:0~1 000×106/L, B:1 001~5 000×106/L, C:>5 000×106/L. The results were statistically analyzed. Results Compared with the results of microscope, the results of Sysmex XN-1000 hematology analyzer showed no statistically significant difference (P>0.05). The correlation coeffi-cients of group A, B, C were 0.993, 0.984, 0.996, with the regression equation of y=1.007x-1.072, y=1.003+19.776x, y=1.241-1 419.365x, respectively. Conclusion The body fluid module of Sysmex XN-1000 hematology analyzer can be used for white blood cell count in clinical CSF and hydrothorax and ascites, with simple operation and reliable results. When the instrument is alarmed on the sample test, we should use manual microscopy review to improve the veracity and reliability of the results.