电离辐射对小鼠外周血单核细胞亚群的影响

目的：探讨电离辐射对小鼠外周血Ly6C⁺和Ly6C^-单核细胞亚群比例和数目的影响，及其在辐射损伤防护中的意义。方法：实验分为4 Gy γ射线照射后第3、5、7、14和21天处理组及对照组，每组10只，雌雄各半。用血球仪检测受照小鼠及其对照组外周血单核细胞数目，用流式细胞术检测受照小鼠外周血Ly6C⁺和Ly6C^-单核细胞亚群的比例，根据单核细胞数目与单核细胞亚群比例的乘积计算出单核细胞亚群的数目。结果：与对照组相比，4 Gy γ射线照射小鼠后第3、5和7天外周血单核细胞Ly6C⁺亚群与Ly6C^-亚群数目显著降低（P<0.01），Ly6C⁺单核亚群数目出现先降低（照后3 d）后升高（照后5 d）再降低（照后7 d）的双相变化。与对照组相比，4 Gy γ射线照射小鼠后第3和第5天外周血Ly6C⁺亚群比例显著升高至90%（P<0.01），Ly6C^-单核亚群的比例显著降低至10%（P<0.01）。与对照组比较，4 Gy γ射线照射小鼠后第14天外周血单核细胞Ly6C⁺亚群数目和比例仍显著降低（P<0.01），第21天外周血Ly6C⁺和Ly6C^-单核亚群的比例和数目基本恢复正常。结论：4 Gy γ射线受照小鼠外周血Ly6C⁺和Ly6C^-单核细胞亚群数目在受照后第1周显著降低，这可能与电离辐射诱导小鼠外周血单核细胞亚群发生凋亡有关。

Effects of ionizing radiation on monocyte subsets in peripheral blood of mice

OBJECTIVE: To explore effects on the percentages and numbers of Ly6C⁺ and Ly6C^- monocyte subsets from peripheral blood of irradiated mice. METHODS: After exposure to 4 Gy γ rays, mice were subdivided into 10 mice per group and evaluated at 3, 5, 7, 14 and 21 days after the irradiation. There were also 10 non-irradiated control mice. The number of monocytes from peripheral blood of irradiated mice was measured by blood cell analysis instrument. The percentage of Ly6C⁺ and Ly6C^- monocyte subsets was detected by flow cytometer. RESULTS: At 3, 5 and 7 days after the irradiation, the numbers of monocyte subsets were significantly decreased (P<0.01) compared to those from the controls. The Ly6C⁺ monocyte subsets fromthe fifth day group were significantly higher than those from the third day group (P<0.01), but significantly decreased (P<0.01) compared to the seventh day group. Compared with control group, the percentages of Ly6C⁺ monocyte subsets from the third and fifth day groups were significantly increased to 90% and the percentage of Ly6C^- monocyte subsets were significantly decreased to 10%. In addition, both the numbers and percentages of monocyte subsets from the fourteenth day group were significantly decreased (P<0.01) compared to the controls. On the other hand, both the numbers and percentages of monocyte subsets recovered at 21 day after irradiation. CONCLUSION: During the first week after irradiation, the numbers of monocyte subsets from peripheral blood of mice were reduced significantly. The decrease may be associated with radiation-induced apoptosis of these monocyte subsets.