湿热环境猫颅脑火器伤早期脑微循环及血脑屏障的变化

目的研究湿热环境对猫颅脑火器伤早期脑微循环的影响，为临床治疗提供实验依据。方法24只成年猫用改良Carey法制作猫颅脑火器伤模型后，随机分为两组，每组12只，放入温度和相对湿度分别为25℃和50％，35℃和85％仿真模拟气候舱。通过脑微血管形态学及通透性和周围组织病理变化的观察，测定生命体征代偿指标、微血管中流动血液流变学特征和血脑屏障的变化来反应脑微循环的变化。结果全部动物无死亡。两组动物在脑微血管形态学及通透性和周围组织病理变化显著；在生命体征指标、血液流变学特征和血脑屏障的损害程度上存在显著差异。结论湿热环境对颅脑火器伤的早期脑微循环有显著影响．是颅脑火器伤的二次创伤。

Changes of cat cerebral microcirculation and blood-brain barrier in early stage of craniocerebral gunshot wound in the hot and humid environment]

OBJECTIVE: To study the changes of cat cerebral microcirculation in early stage after craniocerebral gunshot wound in the hot and humid environment to provide laboratory evidence for clinical treatment of such wound. METHODS: Craniocerebral gunshot wound was induced in 24 cats according to the method described by Carey with modifications, and the cats were placed in a cabin with environmental temperature and humidity of 25 degrees Celsius and 50% (group A), and 35 degrees Celsius and 85% (group B), respectively, to observe the changes in all the indices of cerebral microcirculation. RESULTS: All the cats survived and notable changes occurred in the morphology and permeability of cerebral microvascular along with obvious pathological changes in the brain tissue, and the vital signs, hemorheology and blood-brain barrier were significantly different between groups A and B. CONCLUSION: Hot and humid environment induces obvious changes in cat cerebral microcirculation and blood-brain barrier function in the early stages after craniocerebral gunshot wound.