肾癌中促红细胞生成素产生肝细胞受体A2的表达及其与E-钙黏蛋白、微血管密度的关系

目的 探讨促红细胞生成素产生肝细胞受体A2(Eph A2)在肾癌组织中的表达及其与肿瘤血管生成、E-钙黏蛋白的关系.方法 利用免疫组织化学法检测肾癌组织68例、正常肾组织24例中EphA2、E-钙黏蛋白的表达,并采用CD34抗体标记微血管内皮细胞,计算微血管密度(MVD).分析EphA2、MVD、E-钙黏蛋白与肾癌临床病理特征的关系及其的相关性.结果 肾癌组织中EphA2阳性表达及MVD明显高于正常肾组织,E-钙黏蛋白阳性表达明显低于正常肾组织(均P＜0.01).EphA2在肾癌组织的高分级组、高分期组、淋巴结转移组显著高于低分级组、低分期组和无淋巴结转移组(P＜0.01或＜0.05);MVD在肾癌组织的高分级组、高分期组、淋巴结转移组的表达亦显著高于低分级组、低分期组和无淋巴结转移组(均P＜0.01);MVD值还与肿瘤直径有关(均P＜0.01);E-钙黏蛋白在肾癌组织的高分级组、高分期组、淋巴结转移组显著低于低分级组、低分期组和无淋巴结转移组(P＜0.01或＜0.05);Spearman等级相关分析表明,EphA2蛋白表达与MVD呈显著正相关(r=0.555,P＜0.01),与E-钙黏蛋白蛋白的表达呈显著负相关(r=-0.483,P＜0.01).结论 EphA2蛋白的高表达预示肿瘤恶性程度的增加,其机制可能通过影响或协同E-钙黏蛋白和微血管生成来实现的.

Expression of erythropoietin producing hepatoma receptor in human renal cell carcinoma and its relationship with angiogenesis and E-cadherin

Objective To investigate the expression of erythropoietin producing hepatoma receptor（Eph） A2 and its relationship with angiogenesis and E-cadherin in renal cell carcinoma and the relevance to the clinic pathological features.Methods The expression and quantitative analysis of the EphA2,E-cadherin were examined by immunohistochemistry（IHC）,respectively,in tissues from 68 cases of renal cell carcinoma and from 24 cases of normal kidney.The microvessel density（MVD） was determined by the CD34 antibody labeled microvascular endothelial cells.Results The expression of EphA2 and MVD were distinctly higher,whereas that of E-cadherin was distinctly lower in the carcinomous tissues than those of the normal ones（all P〈0.01）.Significantly increased expression of the EphA2 and MVD and decreased expression of the E-cadherin were detected in carcinomous tissues with higher classification grades,more advanced stages and lymph node metastasis,comparing with the carcinomous tissues with lower grades,less advanced stages and no lymph node metastasis,respectively（P〈0.01 or 〈0.05）.MVD was further related with the diameter of the tumor（P〈0.01）.Spearman correlation and factor analysis shows that the protein expression of the EphA2 was positively associated with MVD and inversely correlated with Ecadherin（r=0.555,P〈0.01;r=-0.483,P〈0.01）.Conclusion The high expression of the EphA2 protein may predict the degree of malignancy of the tumor,possibly through the influence of or the coordination with the E-cadherin and the angiogenesis of microvasculatures.