| DAPI标记骨髓间充质干细胞在胶质瘤模型大鼠脑内的迁徙和定位 |
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| 引用本文： | 于音,杨洪发,邵佳甲,邬巍,朱东,姜涛,郭永川,梁前垒,李衍鑫.DAPI标记骨髓间充质干细胞在胶质瘤模型大鼠脑内的迁徙和定位[J].中国神经再生研究,2010,14(23):4243-4246. |
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| 作者姓名： | 于音 杨洪发 邵佳甲 邬巍 朱东 姜涛 郭永川 梁前垒 李衍鑫 |
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| 作者单位： | 吉林大学白求恩第三临床学院，吉林省长春市 130031,吉林大学白求恩第一临床学院，吉林省长春市 130021,长春职业技术学院，吉林省长春市 130031,吉林大学白求恩第一临床学院，吉林省长春市 130021,吉林大学白求恩第一临床学院，吉林省长春市 130021,吉林大学白求恩第三临床学院，吉林省长春市 130031,吉林大学白求恩第三临床学院，吉林省长春市 130031,吉林大学白求恩第三临床学院，吉林省长春市 130031,吉林大学白求恩第三临床学院，吉林省长春市 130031 |
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| 基金项目： | 课题由长春市科技计划项目（08SF15）资助，项目为人骨髓间充质干细胞在脑损伤修复中的作用研究 |
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| 摘 要： | 背景：骨髓间充质干细胞作为载体细胞行脑内移植治疗胶质瘤，如何证实其为最好的药物载体？
目的：将DAPI标记的大鼠骨髓间充质干细胞移植入模型鼠脑内，观察骨髓间充质干细胞在肿瘤内的迁徙与定位。
方法: 体外培养骨髓间充质干细胞。利用立体定向仪将培养好的C6细胞注入大鼠脑内，建立大鼠脑内胶质瘤模型。将DAPI标记于培养好的骨髓间充质干细胞上，利用微量注射器注入模型鼠脑内；移植后第3，7天处死大鼠，利用共聚焦显微镜观察骨髓间充质干细胞在肿瘤内的分布及与肿瘤细胞的关系。
结果与结论:实验成功建立了大鼠脑内胶质瘤模型。以DAPI标记的骨髓间充质干细胞。在模型鼠脑内主动聚集于肿瘤血管周围，并能与肿瘤细胞发生融合。结果证实骨髓间充质干细胞可以作为肿瘤基因治疗的良好载体。
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| 关 键 词： | 胶质瘤 骨髓间充质干细胞 大鼠 DAPI标记 脑 |
Migration and localization of bone marrow mesenchymal stem cells labeled by DAPI in the brain of glioma rat models |
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| Yu Yin,Yang Hong-f,Shao Jia-ji,Wu Wei,Zhu Dong,Jiang Tao,Guo Yong-chuan,Liang Qian-lei and Li Yan-xin.Migration and localization of bone marrow mesenchymal stem cells labeled by DAPI in the brain of glioma rat models[J].Neural Regeneration Research,2010,14(23):4243-4246. |
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| Authors: | Yu Yin Yang Hong-f Shao Jia-ji Wu Wei Zhu Dong Jiang Tao Guo Yong-chuan Liang Qian-lei and Li Yan-xin |
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| Institution: | Norman Bethune Third Clinical Hospital, Jilin University, Changchun 130031, Jilin Province, China,Norman Bethune First Clinical Hospital, Jilin University, Changchun 130021, Jilin Province, China,College of Occupation Technology, Changchun 130031, Jilin Province, China,Norman Bethune First Clinical Hospital, Jilin University, Changchun 130021, Jilin Province, China,Norman Bethune First Clinical Hospital, Jilin University, Changchun 130021, Jilin Province, China,Norman Bethune Third Clinical Hospital, Jilin University, Changchun 130031, Jilin Province, China,Norman Bethune Third Clinical Hospital, Jilin University, Changchun 130031, Jilin Province, China,Norman Bethune Third Clinical Hospital, Jilin University, Changchun 130031, Jilin Province, China,Norman Bethune Third Clinical Hospital, Jilin University, Changchun 130031, Jilin Province, China |
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| Abstract: | BACKGROUND: Bone marrow mesenchymal stem cells (BMSCs) as vector cells were transplanted in the brain for treating glioma. How to verify BMSCs are best drug carriers.
OBJECTIVE: To observe the migration and localization of BMSCs in tumor following transplantation of rat BMSCs labeled by DAPI in the brain of a rat model.
METHODS: BMSCs were cultured in vitro. Using stereotaxic apparatus, C6 cells were injected into rat brain to establish glioma models. BMSCs labeled by DAPI were injected into the rat brain using a microinjector. The rats were sacrificed at 3 and 7 days following transplantation. The localization of BMSCs in tumor and the relationship with tumor cells were observed under a confocal microscope.
RESULTS AND CONCLUSION: The glioma model in rat brain was established successfully. BMSCs labeled by DAPI can accumulate around tumor vessel actively in rat brain, and can mix together with tumor cell. These suggested that BMSCs are a good carrier of gene therapy. |
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| Keywords: | Glioma Bone marrow Mesenchymal stem cells Rat |
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