CD30+间变性大细胞淋巴瘤细胞系的建立及其生物学特性

由1例非霍奇金淋巴瘤患者的胸水标本通过液体培养法建立了1株CD30^ 间变性大细胞淋巴瘤细胞后，并对其生物学特性进行研究。所建立的细胞系可以在含10%小牛血清的RPMI 1640培养液中持久增殖，倍增时间约为36小时，目前已传代120余次，生长良好，暂命名为SH-1。光镜下细胞形态与典型的CD30^ 间变性大细胞淋巴瘤（anaplastic large cell lymphoma,ALCL）细胞的形态十分类似，电镜下可观察到胸浆内存在较多病毒颗粒。细胞组织化学染色：光镜下过氧化物酶POX阴性，糖原染色PAS阳性，酸性磷酸酶（ACP）呈强阳性；电镜下髓过氧化物酶（MPO）阴性，血小板过氧化物酶（PPO）阴性。EB病毒核心抗原-1（EBNA-1）呈阳性。细胞免疫表型为CD30^ 、CD45^ 、HLA-DR^ ，而EMA、CD34、CD38、CD2、CD3、CD4、CD7、CD8、CD10、CD15、CD19、CD20均呈阴性。染色体分析示SH-1细胞系核型为二倍体或四倍体核型，并具有明显的结构和数量异常，但未见典型t(2;5)。结论：所建立的细胞系为CD30^ ALCL细胞系。

Establishment of a Human CD30+ Anaplastic Large Cell Lymphoma Cell Line and Its Biological Characteristics

A CD30+ anaplastic large cell lymphoma (ALCL) cell line was established from the mononuclear cells isolated from pleural effusion of a patient with non-Hodgkin's lymphoma. The cell line's biological characteristics were analyzed. The results showed that the established cell line could survive and proliferate in RPIM 1640 medium; the Wright-Giemsa-stained cells were exactly similar to malignant cells of CD30+ ALCL in morphology, with many diffuse virus granules in cytoplasm; the cytochemical staining of the cells showed the following reactivity pattern: positive for acid phosphatase (ACP) and periodic acid-Schiff (PAS), negative for peroxidase (POX), myeloperoxidase (MPO) and platelet peroxidase (PPO). The immunoprofile of the cells was positive for CD45, HLA-DR, CD30 and negative for EMA, CD34, CD38, CD2, CD3, CD4, CD7, CD8, CD10, CD15, CD19 and CD20. The cytogenetic analysis showed complicate d qualitative and quantitative abnormality of chromosomes, without typical t(2;5). It is concluded that the established cell line is CD30+ anaplastic large cell lymphoma cell line.