葛根素对运动大鼠血液流变及运动能力的影响

摘要目的：分析葛根素干预对运动训练大鼠血液流变学及运动能力的影响。方法：60只8周龄SD雄性大鼠，30只口服葛根素组和30只对照组，葛根素组再分为10只葛根素安静组，20只葛根素+训练组（10只为运动后即刻组，10只为运动后恢复24h组）；同样30只对照组也分10只安静组，20只训练组（10只为运动后即刻组，10只为运动后恢复24h组），建立力竭游泳训练模型。通过灌服葛根素干预后，观测大鼠力竭游泳时间，测试各组大鼠全血黏度、血浆黏度、红细胞聚集指数、红细胞刚性指数、红细胞压积、红细胞沉降率（血沉）。结果：①安静时：与对照组相比，葛根素组大鼠全血黏度显著降低[(8.41±0.76)，（6.51 ±0.92），P<0.05]，红细胞刚性指数显著降低[(1.23±0.76)，（0.98±0.72），P<0.05]和红细胞聚集指数显著降低[(16.65±2.45)，（14.78±1.55），P<0.05]；②运动后即刻时：与对照训练组相比，葛根素训练组全血黏度显著下降(P<0.05)，血浆黏度显著降低[(1.75±3.3)，（1.58±0.21），P<0.05]，红细胞聚集指数显著下降[(21.37±4.78)，（19.43±5.86），P<0.05]，红细胞刚性指数显著下降[(1.58±0.90)，（1.39±0.99），P<0.05]，红细胞压积显著降低[(0.71±0.45)，（0.57±0.43），P<0.05]；③运动后恢复24h：与对照训练组相比，全血黏度、红细胞聚集指数、红细胞刚性指数、红细胞压积显著降低(P<0.05)，血浆黏度差异无显著性差异[(1.45±0.47)，（1.52±0.26），P>0.05]④游泳至力竭时间：葛根素组游泳时间较对照组有显著延长(P

Experimental study on effect of puerarin on hemorheology and exercise performance in trained rats

Objective:To observe the effects of intervention of puerarin on blood hemorheology and exercise performance of rats after exercise.Method:The 60 male SD rats aged 8 weeks were selected,randomly divided two big groups:puerarin supplement groups and control groups,each group had 30 rats.each big group divided three small groups:the sedentary group,the train immediately group and the train after 24 hours recovery group,each group had 10 rats.Then established the rats exhaust-swimming train model.After puerarin supplement the exhausting-swimming time was recorded,rats’ exercise performances were observed and the blood viscosity,plasma viscosity,aggregability of red blood cell(ARBC),hematocrit(HCT),erythrocyte index of rigidity(EIR),erythrocyte sedimentation rate(ESR)were measured.Result:①On sedentary time:Compared with control group,the whole blood viscosity of puerarin group declined significantly[(8.41±0.76),(6.51±0.92),P<0.05],the EIR declined significantly[(1.23±0.76),(0.98±0.72),P<0.05],the ARBC of puerarin group declined significantly[(16.65±2.45),(14.78±1.55),P<0.05];②On the train immediately:Compared with control group,the blood viscosity of puerarin group declined significantly(P<0.05),the plasma viscosity declined significantly[(1.75±3.3),(1.58±0.21),P<0.05],the ARBC of puerarin group declined significantly[(21.37±4.78),(19.43±5.86),P<0.05],the EIR declined significantly [(1.58±0.90),(1.39±0.99),P<0.05],the HCT of puerarin group declined significantly[(0.71±0.45),(0.57±0.43),P<0.05];③After 24 hours recovery:Compared with control group,the blood viscosity,ARBC,EIR,HCT of puerarin group declined significantly(P<0.05),the plasma viscosity of puerarin group had no significant difference[(1.45±0.47),(1.52±0.26),P>0.05];④ The exhausted swimming time:The swimming time of the puerarin group was longer than the control group(P<0.05),and the Exhaustive Delay Rate Increased to 28%.Conclusion:Puerarin could effectively prolong the exhausted swimming time and improve hemorheological indices.