| 蜈蚣三七有效成分W3的提取分离及其免疫活性的初步研究 |
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| 引用本文： | 邴飞虹,张国斌,苏彦奇. 蜈蚣三七有效成分W3的提取分离及其免疫活性的初步研究[J]. 中国药业, 2010, 19(15): 6-7 |
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| 作者姓名： | 邴飞虹 张国斌 苏彦奇 |
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| 作者单位： | 湖北中医学院中药资源与中药复方重点实验室,湖北,武汉,430065 |
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| 摘 要： | 目的研究蜈蚣三七有效成分W3的提取分离方法及其对小鼠脾淋巴细胞增殖的影响。方法利用硅胶柱和C18色谱柱分离纯化W3,采用四甲基偶氮唑蓝法分别检测蜈蚣三七总皂苷、W3及齐墩果酸分别对小鼠脾淋巴细胞体外增殖反应的影响。结果 W3对正常增殖的淋巴细胞活性无明显影响;剂量不低于0.1μmol/L时,W3对刀豆蛋白诱导的淋巴细胞增殖活性有显著抑制作用（P〈0.05）。结论蜈蚣三七有效成分W3具有较强的免疫活性。
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| 关 键 词： | 蜈蚣三七 有效成分 淋巴细胞增殖 免疫活性 |
Extraction and Separation of Effective Constituent W3 in Flaccid Anemone Rhizome and Its Immunocompetence |
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| Bing Feihong,Zhang Guobin,Su Yanqi. Extraction and Separation of Effective Constituent W3 in Flaccid Anemone Rhizome and Its Immunocompetence[J]. China Pharmaceuticals, 2010, 19(15): 6-7 |
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| Authors: | Bing Feihong Zhang Guobin Su Yanqi |
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| Affiliation: | (Key Laboratory of Chinese Medicine Resources and Chinese Medicine Compound,Hubei College of Traditional Chinese Medicine,Wuhan,Hubei,China 430065) |
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| Abstract: | Objective To study the effect of the effective constituent W3 in flaccid anemone rhizome on lymphopoiesis of mouse spleen.Methods The silicagel column and C18 chromatographic column were used to separate and purify W3.The methyl thiazolyl tetrazolium（MTT） method was adopted to investigate the effects of gensenoside,W3 and oleanolic acid on in vitro lymphopoiesis of mouse spleen respectively.Results W3 had no obvious effect on lymphocyte activity of normal lymphopoiesis.In the dosage of no less than 0.1 μmol /L,W3 had significant inhibitory effect on the lymphopoiesis activity induced by concanavalin（P 0.05）.Conclusion The effective constituent W3 in flaccid anemone rhizome has stronger immunocompetence. |
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| Keywords: | flaccid anemone rhizome effective constituent lymphopoiesis immunocompetence |
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