滤纸干血滴间接荧光抗体试验法诊断阿米巴病的研究

作者于1985年7月～1987年10月,用滤纸干血滴作间接荧光试验诊断31例阿米巴肝脓肿,13例阿米巴痢疾和15例阿米巴包囊携带者,其阳性率分别在100％,69.23％和33.3％,无假阳性和交叉反应。与血清比较无明显差异。该方法诊断阿米巴肝脓肿有较高的特异性、敏感性和重复性。

Application of IFA with Dried Blood Spot on Filter Paper in Diagnosis of Amoebiasis

Using indirect fluorescent antibody assay with dried blood spot on the filter paper for the laboratory diagnosis of entamoebiasjs histolytica in various-manifestion of clinica amoebiasis were studied in 31 cases of amebic liver abscess, 13 cases of amebic dysentery and 15 cases asymptomatic cyst passers.The positive rates of amebic liver abscess, amebic dysentery and asypmtomatic cyst passer were 100%, 69.23% and 33.33% respectively. The sensitivity, specificity and reprodu-cibility of this assay for diagnosis were also studied, no false positive case was found in this test. There was no statistically significant difference when substitued dried blood spot on filter paper for serum in the results of indirect fluorescent antibody assay. No cross reaction against other parasitic infections ap.d other infections were found in this test. The dried blood spot by indirect fluorescent antibody assay is very useful in the diagnosis of amebic liver abscess but less sensistive for amebic dysentery and asymptomatic cyst passers. Specificity and reproducibility of this method were good.