脑脊液14-3-3蛋白分析在评价多发性硬化轴索损害中的临床价值

目的 评价脑脊液14-3-3蛋白与多发性硬化（MS）患者轴索损害之间的关系。方法 从47例MS患者及36例对照中获取脑脊液标本，用免疫印迹法分析脑脊液14-3-3蛋白条带。结果 MS患者脑脊液14-3-3蛋白阳性率为24．5％，急性期起病的患者12例中7例阳性；阳性的患者其扩展的残疾功能状况量表评分均≥2．0分，且〉4．5分的患者阳性率更高。结论 免疫印迹法分析脑脊液14-3-3蛋白对判断MS患者急性期是否出现轴索损害及遗留神经功能缺损有一定的参考价值。

Clinical implication of cerebrospinal fluid 14-3-3 protein analysis in assessing axonal damage of multiple sclerosis

Objective To investigate the relationship between 14-3-3 protein in the cerebrospinal fluid (CSF) and the axonal damage of multiple sclerosis. Methods The 14-3-3 immuno-reactivity was analyzed by immuno-blot analysis in the CSF samples obtained from 47 patients with multiple sclerosis, as well as from 36 controls. Results CSF 14-3-3 reactivity was positive in 24.5% of the MS patients and in 58.3% of the patients within acute period. The more severe the disability of the MS patients as judged by expanded disability status scale, the higher the positive rate of CSF 14-3-3 reactivity. Conclusion Immuno-blot of analysis of 14-3-3 protein in CSF is valuable in assessing axonal damage of multiple sclerosis and could be a useful biomarker to predict neurological impairment.