Neurogenesis of adipose-derived stem cells in hydrogel |
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| Authors: | Xuewei Xie Zhouping Tang Juan Chen Jie Yang Wengao Zeng Na Liu Yongming Liu |
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| Institution: | 1. Department of Neurology, Tongji Hospital, Tongji Medical University, Huazhong University of Science and Technology, Wuhan 430030, China;Department of Neurology, Changzheng Hospital, the Second Military Medical University, Shanghai 200003, China
2. Department of Neurology, Changzheng Hospital, the Second Military Medical University, Shanghai 200003, China |
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| Abstract: | Adipose tissue is a readily available source of adult stem cells with multipotent properties suitable for tissue engineering
and regenerative medical applications. Peptide hydrogel is a novel biomaterial which provides three-dimensional microenvironments
for a variety of cells for tissue grafting. In this study, adipose-derived stem cells (ADSCs) were isolated from rats, seeded
into the peptide hydrogel polymer scaffolds and cultured in Neurobasal (NB) media supplemented with B27, basic fibroblast
growth factor (bFGF) and epidermal growth factor (EGF). Ten days after the culture, some cells were expanded into clonal populations
in which the expression of both Nestin and Brdu was detected but only Brdu expression was detected in the cells that were
not expanded into clonal populations. Our results suggested that ADSCs in peptide hydrogel polymer scaffolds can be induced
to differentiate into cells capable of expressing the neuron-associated markers, self-renewal and self-propagation. |
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| Keywords: | adipose-derived stem cells hydrogel neurogenesis tissue engineering |
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