Correlation of polymorphism of IL-8 and MMP-7 with occurrence and lymph node metastasis of early stage cervical cancer |
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Authors: | Suhui WU Shi LU Huijuan TAO Li ZHANG Weifeng LIN Haixia SHANG Jun XIE |
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Institution: | (Department of Postgraduate, the First Hospital, Shanxi Medical University, Taiyuan 030001, China) ; |
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Abstract: | The IL-8 and MMP-7 genes participate in the carcinogenesis of many malignancies, but the role of both genes in cervical cancer
is not fully elucidated. The aim of this study was to determine the frequency of IL-8 and MMP-7 gene mutations and to assess
their effects on the risk of early stage cervical cancer and lymph node metastasis. The clinical stage and histological grade
of cervical cancer were also studied. The peripheral blood from the patients with early stage cervical cancers and normal
controls was collected and the DNA was extracted. The incidence of IL-8 and MMP-7 gene mutations was assessed by using tetra-primer
amplification refractory mutation system polymerase chain reaction (ARMS PCR) and restriction fragment length polymorphism
(RFLP). The data were statistically analyzed by x2 test. The results showed that: (1) The genotype frequency of IL-8 -251AT and TT was significantly higher in the cervical
cancer group than in the normal control group (OR=2.290 and 2.619 respectively, P=0.001), and it was also higher in the lymphatic metastasis group than that without metastasis (OR=2.917, P=0.035); (2) The frequency of MMP-7 -181G/G genotype was significantly higher in the cervical cancer group and in the lymphatic
metastasis group (P<0.05); (3) The incidence of IL-8 mutation was two times higher in IIa cervical cancer group than in Ib1 and Ib2 cervical
cancer group (P=0.006). For the MMP-7 gene, there was statistically significant difference in the incidence of mutation between the Ib1,
Ib2 and the IIa (P=0.000); (4) Different histological types and different grades of cervical cancer had different incidence of mutations, statistically.
It was suggested that there was significant difference in the genotype of IL-8 -251TT and MMP-7 -181GG polymorphism between
the cervical cancer group and the lymph node metastasis group. Moreover, individuals with IL-8 T allele or MMP-7 G allele
carriers were at significantly higher risk of cervical cancer, particularly the early (IIa) and medium, poorly differentiated
cervical cancer (G2+G3). |
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Keywords: | interleukin-8 matrix metalloproteinases polymorphism cervical cancer |
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