ZrO_2和Ti_6Al_4V对牙龈成纤维细胞凋亡的影响

目的：研究ZrO2、Ti6Al4V对牙龈成纤维细胞的毒性影响,为临床优选材料提供理论依据。方法：分别制作ZrO2、Ti6Al4V试件各10片,并制作浸提液。常规培养细胞并确定细胞接种密度（6×103个/mL）,倒置相差显微镜下观察细胞形态,应用流式细胞仪检测细胞凋亡率（AP%）。结果：ZrO2、Ti6Al4V的AP%值经统计学分析无显著性差异（P〉0.05）。结论：ZrO2对细胞增殖的抑制作用极弱,表现出极佳的生物相容性。

ZrO2 and Ti6Al4V on gingival fibroblast apoptosis.

Objective：Study on ZrO2,Ti6Al4V on the gingival fibroblast toxicity of the impact of preferred materials for clinical theory.Method：Produced ZrO2,Ti6Al4V specimen of the 10,and the production of extracts.Conventional cell culture and to determine the density of cells（6×103 cells/mL）.Under the inverted phase contrast microscope to observe cell morphology,flow cytometry to detect cell apoptosis rate（AP %）.Result：ZrO2,Ti6Al4V value of AP % by statistical analysis,（P 0.05）no significant difference.Conclusion：ZrO2 on cell proliferation inhibition is weak,showing a good biocompatibility.