BD针在重症急性胰腺炎急性肺损伤大鼠模型制备中的应用

目的 探讨BD针在重症急性胰腺炎急性肺损伤研究中大鼠模型建立的改良应用.方法 健康成年雄性SD大鼠40只,随机分为模型组(n=30)、假手术组(n=10),应用BD静脉留置针经十二指肠乳头逆行胰胆管注入4%牛磺胆酸(0.1 mL/ks),建立重症急性胰腺炎急性肺损伤大鼠模型.模型组在建立后3 h、6 h、12 h检测大鼠血淀粉酶、血气分析、腹水量、肺含水量,光镜下进行胰腺和肺组织病变程度评分.假手术组在术后12 h检测上述相关指标.结果 模型组较假手术组血淀粉酶、腹水量、肺含水率、PaCO2、胰腺和肺组织学评分明显升高(P＜0.05).同时PaO2,氧合指数明显降低(P＜0.05).结论 应用BD静脉留置针能有效制备简便、易复制、稳定性好的重症急性胰腺炎急性肺损伤大鼠模型.

BD intravenous catheters used in inducing the model of acute lung injury with severe acute pancreatitis in rats

Objective To investigate the improvement of BD intravenous catheters used in inducing the model of acute lung injury with severe acute pancreatitis in rats.Methods Total 40 healthy adult male SD rats were randomly assigned to 2 groups,SO group(n=10),SAP group(n=30),4% tauroeholate(0.1ml/kg)was injected via retrograde biliopancreatic ducts by using BD intravenous catheters via duodenal papilla.The serum amylase,blood gas analysis,ascites volume,lung water content and the pathological score of pancreas and lung structure under light microscope were recorded when the SAP model were induced in 3 h,6 h and 12 h.The relative data mentioned as above also detected in SO group after 12 h.Results The score of the SAP group was significantly higher than the SO group in blood amylase,ascites volume,lung water content,PaCO2,pancreas and lung histology(P＜0.05).Meanwhile,PaO2,oxygenation index were decreased significantly(P＜0.05).Conclusion It is efficient to establish simple,reproducible and stabile rat model of acute lung injury with severe acute pancreatitis by using BD intravenous catheters.