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维生素K3诱导肝癌细胞SMMC-7721凋亡及其survivin基因表达的变化
引用本文:石亮 林向阳 陈晓东 陶志华. 维生素K3诱导肝癌细胞SMMC-7721凋亡及其survivin基因表达的变化[J]. 临床检验杂志, 2007, 25(3): 189-191
作者姓名:石亮 林向阳 陈晓东 陶志华
作者单位:温州医学院附属第一医院检验科,浙江温州,325000;温州医学院附属第一医院检验科,浙江温州,325000;温州医学院附属第一医院检验科,浙江温州,325000;温州医学院附属第一医院检验科,浙江温州,325000
摘    要:目的 观察维生素K3(VK3)对人肝癌细胞株SMMC-7721凋亡的影响及其survivin基因表达的变化.方法 CCK 8试剂检测VK3对SMMC-7721细胞的增殖抑制作用,Hoechst 33342荧光染色法在激光共聚焦显微镜下观察凋亡细胞核的形态,用流式细胞仪检测细胞凋亡率(Annexin V/PI法),RT-PCR法检测SMMC-7721细胞survivin mRNA的表达水平.结果 (2、5、10、20、25μmol/L)VK3作用SMMC-7721细胞48 h后,生长抑制率分别为33.8%、50.1%、63.9%、78.5%、84.7%;细胞的凋亡率分别是33.28%、63.97%、77.69%、87.30%、92.40%;survivin mRNA在凋亡的SMMC-7721细胞内的表达明显下降.结论 VK3能抑制SMMC-7721细胞增殖,并诱导其发生凋亡,survivin表达水平的下降可能与VK3诱导的SMMC-7721细胞凋亡有关.

关 键 词:维生素K3  细胞凋亡  survivin
文章编号:1001-764X(2007)03-0189-03
收稿时间:2006-08-30
修稿时间:2006-08-302007-02-16

Effect of vitamin K3 on inducing apoptosis in cell line SMMC-7721 of human liver cancer and expression of survivin gene
SHI liang,LIN Xiangyang,CHEN Xiaodong,TAO Zhihua. Effect of vitamin K3 on inducing apoptosis in cell line SMMC-7721 of human liver cancer and expression of survivin gene[J]. Chinese Journal of Clinical Laboratory Science, 2007, 25(3): 189-191
Authors:SHI liang  LIN Xiangyang  CHEN Xiaodong  TAO Zhihua
Affiliation:Department of Laboratory Medicine, The First Affiliated Hospitial of Wenzhou Medical College, Zhejiang, Wenzhou 325000, China
Abstract:Objective To observe the effects of vitamin K3(VK3) on cell growth and apoptosis in the cell line SMMC-7721 of human liver cancer and the changes in expression of survivin gene.Methods The inhibitory effect of VK3 on SMMC-7721 cells was examined by CCK-8 (cell counting kit).Morphological evaluation of apoptosis was performed by Hoechst33342 staining.The amount of apoptotic cell was assayed by flowcytometry.The changes of survivin gene expression were detected by RT-PCR.Results The inhibitory rate of VK3 (at concentrations of 2,5,10,20,25 mmol/L for 48 h) on SMMC-7721 growth was 3.8%,50.1%,63.9%,78.5%,84.7% respectively.The cells in apoptosis were strongly stained by Hoechst33342 compared with the cells in control group.The apoptostic rate of cells was 33.28%,63.97%,77.69%,87.30% and 92.40%.Following exposure to VK3 at the concentration of 2,5,10,20 and 25 mmol/L for 48h,the survivin mRNA expression in SMMC-7721 was downregulated.Conclusion VK3 inhibits the proliferation of SMMC-7721 cells and induce apoptosis.The mechanism may related to survivn gene.
Keywords:Vitamin K3    cell apoptosis    liver cancer   survivin
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