烧伤大鼠血清对大鼠骨髓间充质干细胞基因表达的影响

目的：观察烧伤大鼠血清对体外培养的大鼠骨髓问充质干细胞(MSCs)基因表达的影响。方法：分离Wistar大鼠骨髓，原代培养MSCs，传代后分别用含有体积分数为10％的正常大鼠血清(N组)和烧伤后3天大鼠血清(B组)的F-12培养基培养24h，然后以含有5705种基因的寡核苷酸微阵列检测两组细胞基因表达的差异。结果：两组MSCs对比，有4种基因表达存在明显差异。其中B组类固醇敏感基因-1(Ssg-1)表达减弱，而磷酸二羟基丙酮酰基转移酶(Gnpat)基因、成纤维细胞生长因子4(FGF-4)基因和与骨形成蛋白-2可诱导激酶的序列有部分相同的一条EST表达增强。结论烧伤后大鼠血清刺激MSCs可使其基因表达发生改变，这种改变可能与MSCs受到刺激后参与创面修复有关。

Effects of serum from burned rats on the gene expression in rat bone mesenchymal stem cells

Objective To investigate the effects of serum from burned rats on the gene expression in cultured rat mesenchymal stem cells (MSCs). Methods Bone marrow was extracted from sacrificed Wistar rats, and MSCs were then incubated for 24h in F-12 medium in the presence of normal rat serum (group N) or serum obtained from burned rats 3 days after injury (group B). Total RNA was extracted from both groups.The mRNA was isolated.An Oligo microarray containing 5705 genes was used to compare the differences of gene expression between two groups. Results There were four genes which expressed differently in two groups.In comparison with group N, the expression of steroid sensitive gene 1 was decreased, but that of fibroblast growth factor-4, dihydroxyacetonephosphate acyltransferase and a EST, which was moderately similar to Bmp2-inducible kinase, were increased in group B. Conclusion Serum from burned rats was able to change the gene expression of MSCs, and it might play the key role in wound repair.