枸杞多糖对新生鼠氯化甲基汞慢暴露染毒致成年期生育能力下降的干预作用

目的：探讨早期低剂量接触氯化甲基汞（Methyl Mercury Chloride,MMC）对大鼠生殖功能和生长发育的影响以及枸杞多糖（Lycium Barbarum Polysaccharides,LBP）的早期干预。方法：将60只健康7日龄大鼠随机分为对照组（CON）、MMC组、MMC＋LBP组、LBP组,MMC组和MMC＋LBP组均颈部皮下注射MMC,CON组颈部注射相同剂量的生理盐水,MMC＋LBP组同时喂养LBP,雌雄合笼,自然观察各组体重发育以及生殖繁育能力以及子代仔鼠早期生长发育和存活状况。再将已孕母鼠哺乳期仔鼠染毒,观察母鼠产仔情况。结果：1）MMC组毛色萎缩,精神状态倦怠、动作不活泼、食欲不振。2）MMC组生长较缓慢且随染毒时间的延长而明显。3）MMC组染毒大鼠未产仔,MMC＋LBP组有产仔,但产死率高、出生后存活率低于CON;LBP组产死率较CON低。4）MMC＋LBP组有畸胎,且仔鼠出生体重与正常CON和LBP组仔鼠出生体重有统计学意义（P〈0.01）。5）染毒仔鼠影响其已孕母鼠再次生育。结论：MMC对大鼠有生殖发育毒性,LBP可抑制MMC引起的生殖毒性。

The Intervention Effect of Lycium Barbarum Polysaccharide on Adulthood Lower Fertility Reproduction Induced by Neonatal Exposed to Methylmercury Chloride in Rats

Objective： To detect the toxicity of neonatal exposed to methylmercury chloride on the adulthood reproduction,as well as ear- ly growth and development in rats and the intervention of Lycium barbarum polysaccharide. Methods： Sixty SD of 7 d rats were randomly divided into saline Control,MMC,MMC ＋ LBP,LBP. The natural development,weight growth and fertilities were observed. Healthy pregnant female rats and MMC exposed neonatal rats and were also observed. Results： 1） Compared with the control group,the rats in MMC group was dropping,inactivity,loss of appetite; 2） The weights of rats in MMC group grew slower than in control rats with the ex- posure time dependently; 3） MMC group were infertility after exposure to the methyl mercury. There remained offspring in MMC ＋ LBP group and their after-born death rates were higher than that of the control group,with low birth weights and low survival rates; 4） The weights in MMC ＋ LBP rats were significant lower（ P 0. 01） than that in in control and LBP group; 5） The MMC exposed pups influ- enced the next reproduction of the female rats. Conclusion： Methylmercury chloride has development and reproductive toxicity effects on rats. Lycium barbarum polysaccharides have potential protection against the toxicity roles.