补肾宁心方对小鼠绝经后骨质疏松的影响 |
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引用本文: | 王玉东,李大金,王文君,朱影.补肾宁心方对小鼠绝经后骨质疏松的影响[J].中国中西医结合杂志,2005,25(11):1000-1003. |
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作者姓名: | 王玉东 李大金 王文君 朱影 |
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作者单位: | 1. 上海交通大学附属第六人民医院妇产科,上海,200233 2. 复旦大学附属妇产科研究所生殖免疫室,上海,200011 |
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基金项目: | 中国科学院资助项目,上海市科技攻关项目,上海市卫生局资助项目 |
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摘 要: | 目的 探讨补肾宁心方防治绝经后骨质疏松的作用.方法 建立BALB/c小鼠绝经后骨质疏松模型,灌服补肾宁心方浓缩液(简称补肾方组),并以17 β-雌二醇为阳性对照(简称E2组),以生理盐水为阴性对照(简称模型组).12周后处死,取血测Th1/Th2型细胞因子;取椎骨(L3-4)和左股骨测骨密度(BMD);取右股骨行骨组织形态计量分析;胫骨提取总RNA,半定量RT-PCR分析护骨素(OPG)的mRNA表达.计算子宫对体重的比值,并行子宫组织切片观察.结果 补肾方组的IFN-γ水平较模型组明显升高(P<0.01),而IL-4水平低于模型组(P<0.05).补肾方组与E2组小鼠的BMD均改善,骨小梁面积增加,OPG mRNA表达增加;但补肾方组小鼠的子宫明显小于E2组(P<0.05),与模型组比较差异无显著性.结论 补肾方能够选择性地防治绝经后骨质疏松,对子宫无或仅有轻微的刺激作用.其机制可能与调节Th1/Th2的偏移及提高OPG的表达,抑制破骨细胞的活性有关.
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关 键 词: | 补肾宁心方 绝经后骨质疏松 护骨素 Th1/Th2 mRNA表达 |
收稿时间: | 2004-12-13 |
修稿时间: | 2005-03-12 |
Predominant Effect of Bushen Ningxin Decoction on Postmenopausal Osteoporosisin Mice |
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Authors: | WANG Yu-dong LI Da-jin LI Da-jin |
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Institution: | Laboratory of Reproductive Immunology, Institute of Obstetrics and Gynecology , Fudan University, Shanghai 200011 |
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Abstract: | OBJECTIVE: To investigate the preventive and therapeutic effect of Bushen Ningxin decoction (BSNX) on postmenopausal osteoporosis. METHODS: The BALB/c female mice postmenopausal osteoporosis model was established. The model mice were treated by BSNX, with 17beta-estradiol (E2) and normal saline as positive and negative control, respectively. All mice were sacrificed after 12 weeks' treatment, the serum cytokines Th1/Th2, bone mineral density (BMD) of vertebrae (L3 - 4) and left femur were determined, and morphological quantitative analysis of bone tissue of right femurs was performed and osteoprotegerin (OPG) mRNA expression in tibia was detected by semi-quantitative RT-PCR. The ratio in weight of uterus to body was calculated, and uterine slice was gotten for histological observation. RESULTS: As compared with the negative control group, the level of interferon-gamma (IFN-gamma) was significantly increased (P < 0.01) and interleukin-4 (IL-4) decreased (P < 0.05) in the BSNX treated group. The BMD of mice were improved, area of bone trabecula and OPG mRNA expression were increased in the BSNX treated and E2 treated group (P < 0.01). But the uterus in the former was significantly smaller than that in the latter (P < 0.01), while it was not significantly different to that in the negative control group. CONCLUSION: BSNX can selectively prevent and cure the postmenopausal osteoporosis, it has no or slight stimulation on uterus. The mechanism may relate with its effects in regulating the deviation of Th1/Th2, enhancing the OPG expression and inhibiting the activity of osteoclasts. |
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Keywords: | Th1/Th2 |
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